Structure determination of a human virus by the combination of cryo-EM and X-ray crystallography

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dc.citation.firstpage55en_US
dc.citation.issueNumber2en_US
dc.citation.journalTitleBiophysics Reportsen_US
dc.citation.lastpage68en_US
dc.citation.volumeNumber2en_US
dc.contributor.authorLiu, Zhengen_US
dc.contributor.authorGuu, Tom S.Y.en_US
dc.contributor.authorCao, Jianhaoen_US
dc.contributor.authorLi, Yinyinen_US
dc.contributor.authorCheng, Lingpengen_US
dc.contributor.authorTao, Yizhi Janeen_US
dc.contributor.authorZhang, Jingqiangen_US
dc.date.accessioned2016-12-16T17:50:26Zen_US
dc.date.available2016-12-16T17:50:26Zen_US
dc.date.issued2016en_US
dc.description.abstractVirus 3D atomic structures provide insight into our understanding of viral life cycles and the development of antiviral drugs. X-ray crystallography and cryo-EM have been used to determine the atomic structure of viruses. However, limited availability of biological samples, biosafety issues due to virus infection, and sometimes inherent characteristics of viruses, pose difficulties on combining both methods in determining viral structures. These have made solving the high resolution structure of some medically important viruses very challenging. Here, we describe our recently employed protocols for determining the high-resolution structure of the virus-like particle of hepatitis E virus (HEV), a pathogen of viral hepatitis in human. These protocols include utilizing recombinant baculovirus system to generate sufficient amount of virus particles, single-particle cryo-EM to get an intermediate resolution structure as a phasing model, and X-ray crystallography for final atomic structure determination. Our protocols have solved the hepatitis E virus structure to the resolution of 3.5 Å. The combined methodology is generally applicable to other human infectious viruses.en_US
dc.identifier.citationLiu, Zheng, Guu, Tom S.Y., Cao, Jianhao, et al.. "Structure determination of a human virus by the combination of cryo-EM and X-ray crystallography." <i>Biophysics Reports,</i> 2, no. 2 (2016) Springer: 55-68. http://dx.doi.org/10.1007/s41048-016-0027-2.en_US
dc.identifier.doihttp://dx.doi.org/10.1007/s41048-016-0027-2en_US
dc.identifier.urihttps://hdl.handle.net/1911/93724en_US
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subject.keywordstructure determinationen_US
dc.subject.keywordCryo-EMen_US
dc.subject.keywordX-ray crystallographyen_US
dc.subject.keywordhuman virusen_US
dc.titleStructure determination of a human virus by the combination of cryo-EM and X-ray crystallographyen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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