Recapitulation of normal collagen architecture in embryonic wounded corneas

Simple item page
dc.citation.articleNumber13815en_US
dc.citation.journalTitleScientific Reportsen_US
dc.citation.volumeNumber10en_US
dc.contributor.authorKoudouna, Elenaen_US
dc.contributor.authorSpurlin, James W.en_US
dc.contributor.authorBabushkina, Annaen_US
dc.contributor.authorQuantock, Andrew J.en_US
dc.contributor.authorJester, James V.en_US
dc.contributor.authorLwigale, Peter Y.en_US
dc.date.accessioned2020-11-02T21:50:09Zen_US
dc.date.available2020-11-02T21:50:09Zen_US
dc.date.issued2020en_US
dc.description.abstractWound healing is characterized by cell and extracellular matrix changes mediating cell migration, fibrosis, remodeling and regeneration. We previously demonstrated that chick fetal wound healing shows a regenerative phenotype regarding the cellular and molecular organization of the cornea. However, the chick corneal stromal structure is remarkably complex in the collagen fiber/lamellar organization, involving branching and anastomosing of collagen bundles. It is unknown whether the chick fetal wound healing is capable of recapitulating this developmentally regulated organization pattern. The purpose of this study was to examine the three-dimensional collagen architecture of wounded embryonic corneas, whilst identifying temporal and spatial changes in collagen organization during wound healing. Linear corneal wounds that traversed the epithelial layer, Bowman's layer, and anterior stroma were generated in chick corneas on embryonic day 7. Irregular thin collagen fibers are present in the wounded cornea during the early phases of wound healing. As wound healing progresses, the collagen organization dramatically changes, acquiring an orthogonal arrangement. Fourier transform analysis affirmed this observation and revealed that adjacent collagen lamellae display an angular displacement progressing from the epithelium layer towards the endothelium. These data indicate that the collagen organization of the wounded embryonic cornea recapitulate the native macrostructure.en_US
dc.identifier.citationKoudouna, Elena, Spurlin, James W., Babushkina, Anna, et al.. "Recapitulation of normal collagen architecture in embryonic wounded corneas." <i>Scientific Reports,</i> 10, (2020) Springer Nature: https://doi.org/10.1038/s41598-020-70658-y.en_US
dc.identifier.doihttps://doi.org/10.1038/s41598-020-70658-yen_US
dc.identifier.urihttps://hdl.handle.net/1911/109469en_US
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleRecapitulation of normal collagen architecture in embryonic wounded corneasen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
s41598-020-70658-y.pdf
Size:
2.87 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Faculty Publications
BioSciences Publications