1,4-Dioxane-degrading consortia can be enriched from uncontaminated soils: prevalence of Mycobacterium and soluble di-iron monooxygenase genes

Simple item page
dc.citation.journalTitleMicrobial Biotechnologyen_US
dc.contributor.authorHe, Yaen_US
dc.contributor.authorMathieu, Jacquesen_US
dc.contributor.authorda Silva, Marcio L.B.en_US
dc.contributor.authorLi, Mengyanen_US
dc.contributor.authorAlvarez, Pedro J.J.en_US
dc.date.accessioned2017-10-27T13:26:30Zen_US
dc.date.available2017-10-27T13:26:30Zen_US
dc.date.issued2017en_US
dc.description.abstractTwo bacterial consortia were enriched from uncontaminated soil by virtue of their ability to grow on 1,4-dioxane (dioxane) as a sole carbon and energy source. Their specific dioxane degradation rates at 30°C, pH = 7 (i.e. 5.7 to 7.1 g-dioxane per g-protein per day) were comparable to those of two dioxane-metabolizing archetypes: Pseudonocardia dioxanivoransCB1190 and Mycobacterium dioxanotrophicusPH-06. Based on 16S rRNA sequencing, Mycobacterium was the dominant genus. Acetylene inhibition tests suggest that dioxane degradation was mediated by monooxygenases. However, qPCR analyses targeting the tetrahydrofuran/dioxane monooxygenase gene (thmA/dxmA) (which is, to date, the only sequenced dioxane monooxygenase gene) were negative, indicating that other (as yet unknown) catabolic gene(s) were responsible. DNA sequence analyses also showed threefold to sevenfold enrichment of group 5 and group 6 soluble di-iron monooxygenase (SDIMO) genes relative to the original soil samples. Whereas biodegradation of trace levels of dioxane is a common challenge at contaminated sites, both consortia degraded dioxane at low initial concentrations (300 μg l−1) below detectable levels (5 μg l−1) in bioaugmented microcosms prepared with impacted groundwater. Overall, this work shows that dioxane-degrading bacteria (and the associated natural attenuation potential) exist even in some uncontaminated soils, and may be enriched to broaden bioaugmentation options for sites experiencing insufficient dioxane catabolic capacity.en_US
dc.identifier.citationHe, Ya, Mathieu, Jacques, da Silva, Marcio L.B., et al.. "1,4-Dioxane-degrading consortia can be enriched from uncontaminated soils: prevalence of Mycobacterium and soluble di-iron monooxygenase genes." <i>Microbial Biotechnology,</i> (2017) Wiley: https://doi.org/10.1111/1751-7915.12850.en_US
dc.identifier.digitalHe_et_al-2017-Microbial_Biotechnologyen_US
dc.identifier.doihttps://doi.org/10.1111/1751-7915.12850en_US
dc.identifier.urihttps://hdl.handle.net/1911/97807en_US
dc.language.isoengen_US
dc.publisherWileyen_US
dc.rightsThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.title1,4-Dioxane-degrading consortia can be enriched from uncontaminated soils: prevalence of Mycobacterium and soluble di-iron monooxygenase genesen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
He_et_al-2017.pdf
Size:
839.27 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Faculty Publications
Civil and Environmental Engineering Publications