Clinical training and validation of the LeukoScope: a low-cost, point-of-care device to perform white blood cell and neutrophil counts

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dc.citation.firstpage27324en_US
dc.citation.journalTitleRSC Advancesen_US
dc.citation.lastpage27333en_US
dc.citation.volumeNumber9en_US
dc.contributor.authorMajors, Catherine E.en_US
dc.contributor.authorPawlowski, Michal E.en_US
dc.contributor.authorBurke, Daniel C.en_US
dc.contributor.authorTkaczyk, Tomasz S.en_US
dc.contributor.authorRieber, Alyssaen_US
dc.contributor.authorRichards-Kortum, Rebeccaen_US
dc.contributor.orgBioengineeringen_US
dc.date.accessioned2019-10-23T14:49:56Zen_US
dc.date.available2019-10-23T14:49:56Zen_US
dc.date.issued2019en_US
dc.description.abstractA white blood cell (WBC) count with partial differential is an important clinical laboratory test. However, current methods to perform a WBC count and differential are difficult to use at the point of care or too expensive for use in low-resource settings. To meet this need, we developed the LeukoScope: a low-cost system to measure a WBC and neutrophil count from a single drop of blood at the point of care. The LeukoScope is battery powered and has a sample-to-answer time of <5 minutes. A drop of blood from a finger stick is added to a LeukoScope sample cartridge where pre-dried acridine orange fluorescently stains WBCs. The cartridge is then inserted into the LeukoScope reader where a portable fluorescence microscope captures a color image of the sample, which is analyzed to report results to the user. The LeukoScope system was tested at the point of care using fingerprick samples collected from 105 general oncology patients in Houston, TX. Performance of the LeukoScope was compared to that of a HemoCue WBC DIFF performed using the same fingerprick sample; clinical laboratory analysis of a venous blood draw was used as the gold standard in all cases. Bland–Altman analysis showed that the LeukoScope and HemoCue WBC DIFF had similar accuracy for measurement of WBC and neutrophil counts as compared to the gold standard. Seven out of eight patients with abnormal WBC count values were correctly identified using the LeukoScope, while six out of eight were correctly identified using the HemoCue WBC DIFF. Five out of six patients with abnormal neutrophil counts were correctly identified using the LeukoScope, while six of six were correctly identified using the HemoCue WBC DIFF.en_US
dc.identifier.citationMajors, Catherine E., Pawlowski, Michal E., Burke, Daniel C., et al.. "Clinical training and validation of the LeukoScope: a low-cost, point-of-care device to perform white blood cell and neutrophil counts." <i>RSC Advances,</i> 9, (2019) Royal Society of Chemistry: 27324-27333. https://doi.org/10.1039/C9RA06433H.en_US
dc.identifier.doihttps://doi.org/10.1039/C9RA06433Hen_US
dc.identifier.urihttps://hdl.handle.net/1911/107485en_US
dc.language.isoengen_US
dc.publisherRoyal Society of Chemistryen_US
dc.rightsThis article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.en_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc/3.0/en_US
dc.titleClinical training and validation of the LeukoScope: a low-cost, point-of-care device to perform white blood cell and neutrophil countsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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