Evaluation of cell-laden polyelectrolyte hydrogels incorporating poly(l-Lysine) for applications in cartilage tissue engineering

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dc.citation.firstpage332en_US
dc.citation.journalTitleBiomaterialsen_US
dc.citation.lastpage346en_US
dc.citation.volumeNumber83en_US
dc.contributor.authorLam, Johnnyen_US
dc.contributor.authorClark, Elisa C.en_US
dc.contributor.authorFong, Eliza L.S.en_US
dc.contributor.authorLee, Esther J.en_US
dc.contributor.authorLu, Stevenen_US
dc.contributor.authorTabata, Yasuhikoen_US
dc.contributor.authorMikos, Antonios G.en_US
dc.date.accessioned2017-05-09T20:16:06Zen_US
dc.date.available2017-05-09T20:16:06Zen_US
dc.date.issued2016en_US
dc.description.abstractTo address the lack of reliable long-term solutions for cartilage injuries, strategies in tissue engineering are beginning to leverage developmental processes to spur tissue regeneration. This study focuses on the use of poly(l-lysine) (PLL), previously shown to up-regulate mesenchymal condensation during developmental skeletogenesis inﾠvitro, as an early chondrogenic stimulant of mesenchymal stem cells (MSCs). We characterized the effect of PLL incorporation on the swelling and degradation of oligo(poly(ethylene) glycol) fumarate) (OPF)-based hydrogels as functions of PLL molecular weight and dosage. Furthermore, we investigated the effect of PLL incorporation on the chondrogenic gene expression of hydrogel-encapsulated MSCs. The incorporation of PLL resulted in early enhancements of type II collagen and aggrecan gene expression and type II/type I collagen expression ratios when compared to blank controls. The presentation of PLL to MSCs encapsulated in OPF hydrogels also enhanced N-cadherin gene expression under certain culture conditions, suggesting that PLL may induce the expression of condensation markers in synthetic hydrogel systems. In summary, PLL can function as an inductive factor that primes the cellular microenvironment for early chondrogenic gene expression but may require additional biochemical factors for the generation of fully functional chondrocytes.en_US
dc.identifier.citationLam, Johnny, Clark, Elisa C., Fong, Eliza L.S., et al.. "Evaluation of cell-laden polyelectrolyte hydrogels incorporating poly(l-Lysine) for applications in cartilage tissue engineering." <i>Biomaterials,</i> 83, (2016) Elsevier: 332-346. https://doi.org/10.1016/j.biomaterials.2016.01.020.en_US
dc.identifier.doihttps://doi.org/10.1016/j.biomaterials.2016.01.020en_US
dc.identifier.urihttps://hdl.handle.net/1911/94214en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.en_US
dc.subject.keywordhydrogelen_US
dc.subject.keywordpoly(L-lysine)en_US
dc.subject.keywordmesenchymal stem cellsen_US
dc.subject.keywordcondensationen_US
dc.subject.keywordchondrogenic differentiationen_US
dc.subject.keywordcartilage tissue engineeringen_US
dc.titleEvaluation of cell-laden polyelectrolyte hydrogels incorporating poly(l-Lysine) for applications in cartilage tissue engineeringen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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