Perfusable cell-laden matrices to guide patterning of vascularization in vivo

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dc.citation.firstpage461
dc.citation.journalTitleBiomaterials Science
dc.citation.lastpage471
dc.citation.volumeNumber11
dc.contributor.authorParkhideh, Siavash
dc.contributor.authorCalderon, Gisele A.
dc.contributor.authorJanson, Kevin D.
dc.contributor.authorMukherjee, Sudip
dc.contributor.authorMai, A. Kristen
dc.contributor.authorDoerfert, Michael D.
dc.contributor.authorYao, Zhuoran
dc.contributor.authorSazer, Daniel W.
dc.contributor.authorVeiseh, Omid
dc.date.accessioned2023-02-16T20:32:55Z
dc.date.available2023-02-16T20:32:55Z
dc.date.issued2023
dc.description.abstractThe survival and function of transplanted tissue engineered constructs and organs require a functional vascular network. In the body, blood vessels are organized into distinct patterns that enable optimal nutrient delivery and oxygen exchange. Mimicking these same patterns in engineered tissue matrices is a critical challenge for cell and tissue transplantation. Here, we leverage bioprinting to assemble endothelial cells in to organized networks of large (>100 μm) diameter blood vessel grafts to enable spatial control of vessel formation in vivo. Acellular PEG/GelMA matrices with perfusable channels were bioprinted and laminar flow was confirmed within patterned channels, beneficial for channel endothelialization and consistent wall shear stress for endothelial maturation. Next, human umbilical vein endothelial cells (HUVECs) were seeded within the patterned channel and maintained under perfusion culture for multiple days, leading to cell–cell coordination within the construct in vitro. HUVEC and human mesenchymal stromal cells (hMSCs) were additionally added to bulk matrix to further stimulate anastomosis of our bioprinted vascular grafts in vivo. Among multiple candidate matrix designs, the greatest degree of biomaterial vascularization in vivo was seen within matrices fabricated with HUVECs and hMSCs encapsulated within the bulk matrix and HUVECs lining the walls of the patterned channels, dubbed design M-C_E. For this lead design, vasculature was detected within the endothelialized, perfusable matrix channels as early as two weeks and αSMA+ CD31+ vessels greater than 100 μm in diameter had formed by eight weeks, resulting in durable and mature vasculature. Notably, vascularization occurred within the endothelialized, bioprinted channels of the matrix, demonstrating the ability of bioprinted perfusable structures to guide vascularization patterns in vivo. The ability to influence vascular patterning in vivo can contribute to the future development of vascularized tissues and organs.
dc.identifier.citationParkhideh, Siavash, Calderon, Gisele A., Janson, Kevin D., et al.. "Perfusable cell-laden matrices to guide patterning of vascularization in vivo." <i>Biomaterials Science,</i> 11, (2023) Royal Society of Chemistry: 461-471. https://doi.org/10.1039/D2BM01200F.
dc.identifier.doihttps://doi.org/10.1039/D2BM01200F
dc.identifier.urihttps://hdl.handle.net/1911/114458
dc.language.isoeng
dc.publisherRoyal Society of Chemistry
dc.rightsThis is an author's post-print. The published article is copyrighted by the Royal Society of Chemistry.
dc.titlePerfusable cell-laden matrices to guide patterning of vascularization in vivo
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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