Stem cell–homing hydrogel-based miR-29b-5p delivery promotes cartilage regeneration by suppressing senescence in an osteoarthritis rat model

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dc.citation.articleNumbereabk0011en_US
dc.citation.issueNumber13en_US
dc.citation.journalTitleScience Advancesen_US
dc.citation.volumeNumber8en_US
dc.contributor.authorZhu, Jinjinen_US
dc.contributor.authorYang, Shuhuien_US
dc.contributor.authorQi, Yadongen_US
dc.contributor.authorGong, Zheen_US
dc.contributor.authorZhang, Haitaoen_US
dc.contributor.authorLiang, Kaiyuen_US
dc.contributor.authorShen, Panyangen_US
dc.contributor.authorHuang, Yin-Yuanen_US
dc.contributor.authorZhang, Zheen_US
dc.contributor.authorYe, Weilongen_US
dc.contributor.authorYue, Leien_US
dc.contributor.authorFan, Shunwuen_US
dc.contributor.authorShen, Shuyingen_US
dc.contributor.authorMikos, Antonios G.en_US
dc.contributor.authorWang, Xiumeien_US
dc.contributor.authorFang, Xiangqianen_US
dc.date.accessioned2022-04-15T14:45:41Zen_US
dc.date.available2022-04-15T14:45:41Zen_US
dc.date.issued2022en_US
dc.description.abstractOsteoarthritis (OA) is a common joint disease characterized by progressive loss of cartilage and reduction in lubricating synovial fluid, which lacks effective treatments currently. Here, we propose a hydrogel-based miRNA delivery strategy to rejuvenate impaired cartilage by creating a regenerative microenvironment to mitigate chondrocyte senescence that mainly contributes to cartilage breakdown during OA development. An aging-related miRNA, miR-29b-5p, was first found to be markedly down-regulated in OA cartilage, and their up-regulation suppressed the expression of matrix metalloproteinases and senescence-associated genes (P16INK4a/P21) via ten-eleven-translocation enzyme 1 (TET1). An injectable bioactive self-assembling peptide nanofiber hydrogel was applied to deliver agomir-29b-5p, which was functionalized by conjugating a stem cell–homing peptide SKPPGTSS for endogenous synovial stem cell recruitment simultaneously. Sustained miR-29b-5p delivery and recruitment of synovial stem cells and their subsequent differentiation into chondrocytes led to successful cartilage repair and chondrocyte rejuvenation. This strategy enables miRNA-based therapeutic modality to become a viable alternative for surgery in OA treatment.en_US
dc.identifier.citationZhu, Jinjin, Yang, Shuhui, Qi, Yadong, et al.. "Stem cell–homing hydrogel-based miR-29b-5p delivery promotes cartilage regeneration by suppressing senescence in an osteoarthritis rat model." <i>Science Advances,</i> 8, no. 13 (2022) AAAS: https://doi.org/10.1126/sciadv.abk0011.en_US
dc.identifier.digitalsciadv-abk0011en_US
dc.identifier.doihttps://doi.org/10.1126/sciadv.abk0011en_US
dc.identifier.urihttps://hdl.handle.net/1911/112100en_US
dc.language.isoengen_US
dc.publisherAAASen_US
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.en_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/en_US
dc.titleStem cell–homing hydrogel-based miR-29b-5p delivery promotes cartilage regeneration by suppressing senescence in an osteoarthritis rat modelen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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