In situ vascularization of injectable fibrin/poly(ethylene glycol) hydrogels by human amniotic fluid-derived stem cells

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dc.citation.journalTitleJournal of Biomedical Materials Research Part Aen_US
dc.contributor.authorBenavides, Omar M.en_US
dc.contributor.authorBrooks, Abigail R.en_US
dc.contributor.authorCho, Sung Kyungen_US
dc.contributor.authorConnell, Jennifer Petscheen_US
dc.contributor.authorRuano, Rodrigoen_US
dc.contributor.authorJacot, Jeffrey G.en_US
dc.contributor.orgBioengineeringen_US
dc.date.accessioned2015-02-27T21:50:06Zen_US
dc.date.available2015-02-27T21:50:06Zen_US
dc.date.issued2015en_US
dc.description.abstractOne of the greatest challenges in regenerative medicine is generating clinically relevant engineered tissues with functional blood vessels. Vascularization is a key hurdle faced in designing tissue constructs larger than the in vivo limit of oxygen diffusion. In this study, we utilized fibrin-based hydrogels to serve as a foundation for vascular formation, poly(ethylene glycol) (PEG) to modify fibrinogen and increase scaffold longevity, and human amniotic fluid-derived stem cells (AFSC) as a source of vascular cell types (AFSC-EC). AFSC hold great potential for use in regenerative medicine strategies, especially those involving autologous congenital applications, and we have shown previously that AFSC-seeded fibrin-PEG hydrogels have the potential to form three-dimensional vascular-like networks in vitro. We hypothesized that subcutaneously injecting these hydrogels in immunodeficient mice would both induce a fibrin-driven angiogenic host response and promote in situ AFSC-derived neovascularization. Two weeks postinjection, hydrogels were sectioned, and the following was demonstrated: the average maximum invasion distance of host murine cells into the subcutaneous fibrin/PEG scaffold was 147 ± 90 µm after 1 week and 395 ± 138 µm after 2 weeks; the average number of cell-lined lumen per square millimeter was significantly higher in hydrogels seeded with stem cells or cocultures containing stem cells (MSC, 36.5 ± 11.4; AFSC, 47.0 ± 18.9; AFSC/AFSC-EC, 32.8 ± 11.6; and MSC/HUVEC, 43.1 ± 25.1) versus endothelial cell types alone (AFSC-EC, 9.7 ± 6.1; HUVEC, 14.2 ± 8.8); and a subset of these lumen were characterized by the presence of red blood cells. Select areas of cell-seeded hydrogels contained CD31+ lumen surrounded by α-smooth muscle cell support cells, whereas control hydrogels with no cells only showed infiltration of α-smooth muscle cell–positive host cells.en_US
dc.identifier.citationBenavides, Omar M., Brooks, Abigail R., Cho, Sung Kyung, et al.. "In situ vascularization of injectable fibrin/poly(ethylene glycol) hydrogels by human amniotic fluid-derived stem cells." <i>Journal of Biomedical Materials Research Part A,</i> (2015) Wiley: http://dx.doi.org/10.1002/jbm.a.35402.en_US
dc.identifier.doihttp://dx.doi.org/10.1002/jbm.a.35402en_US
dc.identifier.urihttps://hdl.handle.net/1911/79045en_US
dc.language.isoengen_US
dc.publisherWileyen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wileyen_US
dc.subject.keywordamniotic fluid-derived stem cellsen_US
dc.subject.keywordvasculogenesisen_US
dc.subject.keywordhydrogelsen_US
dc.subject.keywordfibrinen_US
dc.subject.keywordangiogenesisen_US
dc.titleIn situ vascularization of injectable fibrin/poly(ethylene glycol) hydrogels by human amniotic fluid-derived stem cellsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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