Lateral Flow-Based Skin Patch for Rapid Detection of Protein Biomarkers in Human Dermal Interstitial Fluid

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dc.citation.firstpage5792en_US
dc.citation.issueNumber11en_US
dc.citation.journalTitleACS Sensorsen_US
dc.citation.lastpage5801en_US
dc.citation.volumeNumber9en_US
dc.contributor.authorWilkirson, Elizabeth C.en_US
dc.contributor.authorLi, Danikaen_US
dc.contributor.authorLillehoj, Peter B.en_US
dc.date.accessioned2025-01-09T20:17:05Zen_US
dc.date.available2025-01-09T20:17:05Zen_US
dc.date.issued2024en_US
dc.description.abstractRapid diagnostic tests (RDTs) offer valuable diagnostic information in a quick, easy-to-use and low-cost format. While RDTs are one of the most commonly used tools for in vitro diagnostic testing, they require the collection of a blood sample, which is painful, poses risks of infection and can lead to complications. We introduce a blood-free point-of-care diagnostic test for the rapid detection of protein biomarkers in dermal interstitial fluid (ISF). This device consists of a lateral flow immunochromatographic assay (LFIA) integrated within a microfluidic skin patch. ISF is collected from the skin using a microneedle array and vacuum-assisted extraction system integrated in the patch, and transported through the lateral flow strip via surface tension. Using this skin patch platform, we demonstrate in situ detection of anti-tetanus toxoid IgG and SARS-CoV-2 neutralizing antibodies, which could be accurately detected in human ISF in <20 min. We envision that this device can be readily modified to detect other protein biomarkers in dermal ISF, making it a promising tool for rapid diagnostic testing.en_US
dc.identifier.citationWilkirson, E. C., Li, D., & Lillehoj, P. B. (2024). Lateral Flow-Based Skin Patch for Rapid Detection of Protein Biomarkers in Human Dermal Interstitial Fluid. ACS Sensors, 9(11), 5792–5801. https://doi.org/10.1021/acssensors.4c00956en_US
dc.identifier.digitalwilkirson-et-al-2024en_US
dc.identifier.doihttps://doi.org/10.1021/acssensors.4c00956en_US
dc.identifier.urihttps://hdl.handle.net/1911/118153en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsExcept where otherwise noted, this work is licensed under a Creative Commons Attribution (CC BY) license. Permission to reuse, publish, or reproduce the work beyond the terms of the license or beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subject.keywordlateral flow immunoassayen_US
dc.subject.keywordmicrofluidicen_US
dc.subject.keywordinterstitial fluiden_US
dc.subject.keywordskinen_US
dc.subject.keyworddiagnosticen_US
dc.subject.keywordtetanusen_US
dc.subject.keywordSARS-CoV-2en_US
dc.titleLateral Flow-Based Skin Patch for Rapid Detection of Protein Biomarkers in Human Dermal Interstitial Fluiden_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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