Expression of pro- and anti-angiogenic factors during the formation of the periocular vasculature and development of the avian cornea

Simple item page
dc.citation.firstpage738
dc.citation.issueNumber6
dc.citation.journalTitleDevelopmental Dynamics
dc.citation.lastpage751
dc.citation.volumeNumber242
dc.contributor.authorKwiatkowski, Sam
dc.contributor.authorMunjaal, Ravi P.
dc.contributor.authorLee, Teresa
dc.contributor.authorLwigale, Peter Y.
dc.date.accessioned2014-10-08T21:25:35Z
dc.date.available2014-10-08T21:25:35Z
dc.date.issued2013
dc.description.abstractBackground: During embryonic development, endothelial precursor cells (angioblasts) migrate relatively long distances to form the primary vascular plexus. The migratory behavior of angioblasts and localization of the primitive blood vessels is tightly regulated by pro-angiogenic and anti-angiogenic factors encountered in the embryonic environment. Despite the importance of corneal avascularity to proper vision, it is not known when avascularity is established in the developing cornea and how pro- and anti-angiogenic factors regulate this process. Results and Discussion: Using Tg(tie1:H2B:eYFP) transgenic quail embryos to visualize fluorescently labeled angioblasts, we show that the presumptive cornea remains avascular despite the invasion of cells from the periocular region where migratory angioblasts reside and form the primary vasculature. Semiquantitative reverse transcriptase polymerase chain reaction analysis and spatiotemporal examination of gene expression revealed that pro- and anti-angiogenic factors were expressed in patterns indicating their potential roles in angioblast guidance. Conclusions: Our findings show for the first time that chick corneal avascularity is established and maintained during development as the periocular vasculature forms. We also identify potential candidate pro- and anti-angiogenic factors that may play crucial roles during vascular patterning in the anterior eye.
dc.identifier.citationKwiatkowski, Sam, Munjaal, Ravi P., Lee, Teresa, et al.. "Expression of pro- and anti-angiogenic factors during the formation of the periocular vasculature and development of the avian cornea." <i>Developmental Dynamics,</i> 242, no. 6 (2013) John Wiley & Sons, Inc.: 738-751. http://dx.doi.org/10.1002/dvdy.23956.
dc.identifier.doihttp://dx.doi.org/10.1002/dvdy.23956
dc.identifier.urihttps://hdl.handle.net/1911/77451
dc.language.isoeng
dc.publisherJohn Wiley & Sons, Inc.
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
dc.subject.keywordpro-angiogenic
dc.subject.keywordanti-angiogenic
dc.subject.keywordangioblast
dc.subject.keywordvasculogenesis
dc.subject.keywordocular development
dc.subject.keywordperiocular vasculature
dc.titleExpression of pro- and anti-angiogenic factors during the formation of the periocular vasculature and development of the avian cornea
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
dvdy23956.pdf
Size:
1.32 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Faculty Publications
Biochemistry and Cell Biology Publications