Remodeling of ECM patch into functional myocardium in an ovine model: A pilot study

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dc.citation.journalTitleJournal of Biomedical Materials Research Part B: Applied Biomaterialsen_US
dc.contributor.authorScully, Brandi B.en_US
dc.contributor.authorFan, Chrisen_US
dc.contributor.authorGrigoryan, Bagraten_US
dc.contributor.authorJacot, Jeffrey G.en_US
dc.contributor.authorVick, G. Wesley IIIen_US
dc.contributor.authorKim, Jeffrey J.en_US
dc.contributor.authorFraser, Charles D.en_US
dc.contributor.authorGrande-Allen, K. Janeen_US
dc.contributor.authorMorales, David L.S.en_US
dc.date.accessioned2015-10-01T15:40:10Zen_US
dc.date.available2015-10-01T15:40:10Zen_US
dc.date.issued2015en_US
dc.description.abstractBackground: Previous studies have demonstrated that surgical patches comprised of small intestinal submucosa-derived extracellular matrix (ECM) have biological remodeling potential. This pilot study investigated histological, mechanical, and bioelectrical properties of an ECM patch implanted in the ovine right-ventricular outflow tract (RVOT). Materials and Methods: ECM patches (2 × 2 cm2) were implanted in four Western Range sheep (wether males, 37–49 kg, age <1 year) and explanted at 5 months (n = 2) and 8 months (n = 2). In vivo analysis included epicardial echocardiography and contact electrical mapping. Optical mapping was used to map electrical activity of two hearts on a Langendorff preparation. Mechanical testing quantified stiffness. Histological stains characterized structure, neovascularization, and calcification; immunohistochemistry (IHC) assessed cell phenotype. Results: In vivo analysis showed that ECM patch tissue was contractile by M-mode and two-dimensional echocardiographic evaluation. In vivo electrical mapping, and optical mapping confirmed that ECM conducted an organized electrical signal. Mechanical testing of native and ECM patched RVOT tissue showed an elastic modulus of the implanted patch comparable to native tissue stiffness. Conclusions: At 5 and 8 months, the ECM had undergone extracellular matrix remodeling and neovascularization without calcification. The ECM was populated with locally aligned muscle cells positive for sarcomeric alpha-actinin, CD45, and troponin I and T. In sheep, the ECM patch appears to have the potential of remodeling to resemble native, functional ventricular tissue as evidenced by histological, mechanical, and electrical properties.en_US
dc.identifier.citationScully, Brandi B., Fan, Chris, Grigoryan, Bagrat, et al.. "Remodeling of ECM patch into functional myocardium in an ovine model: A pilot study." <i>Journal of Biomedical Materials Research Part B: Applied Biomaterials,</i> (2015) Wiley: http://dx.doi.org/10.1002/jbm.b.33484.en_US
dc.identifier.doihttp://dx.doi.org/10.1002/jbm.b.33484en_US
dc.identifier.urihttps://hdl.handle.net/1911/81857en_US
dc.language.isoengen_US
dc.publisherWileyen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley.en_US
dc.subject.keywordmyocardiumen_US
dc.subject.keywordremodelingen_US
dc.subject.keywordangiogenesis/neovascularizationen_US
dc.subject.keywordanimal modelen_US
dc.subject.keywordcardiovascularen_US
dc.titleRemodeling of ECM patch into functional myocardium in an ovine model: A pilot studyen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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