Enhancement of performance in porous bead-based microchip sensors: effects of chip geometry on bio-agent capture

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dc.citation.firstpage48195en_US
dc.citation.journalTitleRSC Advancesen_US
dc.citation.lastpage48206en_US
dc.citation.volumeNumber5en_US
dc.contributor.authorKulla, Elionaen_US
dc.contributor.authorChou, Jieen_US
dc.contributor.authorSimmons, Glennonen_US
dc.contributor.authorWong, Jorgeen_US
dc.contributor.authorMcRae, Michael P.en_US
dc.contributor.authorPatel, Rushien_US
dc.contributor.authorFloriano, Pierre N.en_US
dc.contributor.authorChristodoulides, Nicolaosen_US
dc.contributor.authorLeach, Robin J.en_US
dc.contributor.authorThompson, Ian M.en_US
dc.contributor.authorMcDevitt, John T.en_US
dc.date.accessioned2016-10-21T17:01:00Zen_US
dc.date.available2016-10-21T17:01:00Zen_US
dc.date.issued2015en_US
dc.description.abstractMeasuring low concentrations of clinically-important biomarkers using porous bead-based lab-on-a-chip (LOC) platforms is critical for the successful implementation of point-of-care (POC) devices. One way to meet this objective is to optimize the geometry of the bead holder, referred to here as a micro-container. In this work, two geometric micro-containers were explored, the inverted pyramid frustum (PF) and the inverted clipped pyramid frustum (CPF). Finite element models of this bead array assay system were developed to optimize the micro-container and bead geometries for increased pressure, to increase analyte capture in porous bead-based fluorescence immunoassays. Custom micro-milled micro-container structures containing an inverted CPF geometry resulted in a 28% reduction in flow-through regions from traditional anisotropically-etched pyramidal geometry derived from Si-111 termination layers. This novel "reduced flow-through" design resulted in a 33% increase in analyte penetration into the bead and twofold increase in fluorescence signal intensity as demonstrated with C-Reactive Protein (CRP) antigen, an important biomarker of inflammation. A consequent twofold decrease in the limit of detection (LOD) and the limit of quantification (LOQ) of a proof-of-concept assay for the free isoform of Prostate-Specific Antigen (free PSA), an important biomarker for prostate cancer detection, is also presented. Furthermore, a 53% decrease in the bead diameter is shown to result in a 160% increase in pressure and 2.5-fold increase in signal, as estimated by COMSOL models and confirmed experimentally by epi-fluorescence microscopy. Such optimizations of the bead micro-container and bead geometries have the potential to significantly reduce the LODs and reagent costs for spatially programmed bead-based assay systems of this type.en_US
dc.identifier.citationKulla, Eliona, Chou, Jie, Simmons, Glennon, et al.. "Enhancement of performance in porous bead-based microchip sensors: effects of chip geometry on bio-agent capture." <i>RSC Advances,</i> 5, (2015) Royal Society of Chemistry: 48195-48206. http://dx.doi.org/10.1039/C5RA07910Aﾠ.en_US
dc.identifier.doihttp://dx.doi.org/10.1039/C5RA07910Aﾠen_US
dc.identifier.urihttps://hdl.handle.net/1911/91972en_US
dc.language.isoengen_US
dc.publisherRoyal Society of Chemistryen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the Royal Society of Chemistry.en_US
dc.subject.keywordlab-on-a-chipen_US
dc.subject.keywordbeadsen_US
dc.subject.keywordbiochipsen_US
dc.subject.keywordgeometryen_US
dc.subject.keywordhot embossingen_US
dc.subject.keywordimmunoassaysen_US
dc.subject.keywordmicrofluidicsen_US
dc.subject.keywordoptimizationen_US
dc.subject.keywordpoint-of-careen_US
dc.subject.keywordprostate specific antigenen_US
dc.subject.keywordthermoplasticsen_US
dc.titleEnhancement of performance in porous bead-based microchip sensors: effects of chip geometry on bio-agent captureen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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