Triple reassortment increases compatibility among viral ribonucleoprotein genes of contemporary avian and human influenza A viruses

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dc.citation.articleNumbere1009962en_US
dc.citation.issueNumber10en_US
dc.citation.journalTitlePLOS Pathogensen_US
dc.citation.volumeNumber17en_US
dc.contributor.authorWaters, Kaitlynen_US
dc.contributor.authorGao, Chengen_US
dc.contributor.authorYkema, Matthewen_US
dc.contributor.authorHan, Leien_US
dc.contributor.authorVoth, Lyndenen_US
dc.contributor.authorTao, Yizhi Janeen_US
dc.contributor.authorWan, Xiu-Fengen_US
dc.date.accessioned2021-10-21T17:53:25Zen_US
dc.date.available2021-10-21T17:53:25Zen_US
dc.date.issued2021en_US
dc.description.abstractCompatibility among the influenza A virus (IAV) ribonucleoprotein (RNP) genes affects viral replication efficiency and can limit the emergence of novel reassortants, including those with potential pandemic risks. In this study, we determined the polymerase activities of 2,451 RNP reassortants among three seasonal and eight enzootic IAVs by using a minigenome assay. Results showed that the 2009 H1N1 RNP are more compatible with the tested enzootic RNP than seasonal H3N2 RNP and that triple reassortment increased such compatibility. The RNP reassortants among 2009 H1N1, canine H3N8, and avian H4N6 IAVs had the highest polymerase activities. Residues in the RNA binding motifs and the contact regions among RNP proteins affected polymerase activities. Our data indicates that compatibility among seasonal and enzootic RNPs are selective, and enzoosis of multiple strains in the animal-human interface can facilitate emergence of an RNP with increased replication efficiency in mammals, including humans.en_US
dc.identifier.citationWaters, Kaitlyn, Gao, Cheng, Ykema, Matthew, et al.. "Triple reassortment increases compatibility among viral ribonucleoprotein genes of contemporary avian and human influenza A viruses." <i>PLOS Pathogens,</i> 17, no. 10 (2021) Public Library of Science: https://doi.org/10.1371/journal.ppat.1009962.en_US
dc.identifier.digitaljournal-ppat-1009962en_US
dc.identifier.doihttps://doi.org/10.1371/journal.ppat.1009962en_US
dc.identifier.urihttps://hdl.handle.net/1911/111591en_US
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleTriple reassortment increases compatibility among viral ribonucleoprotein genes of contemporary avian and human influenza A virusesen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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