Adaptation of Enterococcus faecalis to Daptomycin Reveals an Ordered Progression to Resistance

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dc.citation.firstpage5373
dc.citation.issueNumber11
dc.citation.journalTitleAntimicrobial Agents and Chemotherapy
dc.citation.lastpage5383
dc.citation.volumeNumber57
dc.contributor.authorMiller, Corwin A.
dc.contributor.authorKong, Jiayi
dc.contributor.authorTran, Truc T.
dc.contributor.authorArias, Cesar A.
dc.contributor.authorSaxer, Gerda
dc.contributor.authorShamoo, Yousif
dc.date.accessioned2014-10-06T17:18:03Z
dc.date.available2014-10-06T17:18:03Z
dc.date.issued2013
dc.description.abstractWith increasing numbers of hospital-acquired antibiotic resistant infections each year and staggering health care costs, there is a clear need for new antimicrobial agents, as well as novel strategies to extend their clinical efficacy. While genomic studies have provided a wealth of information about the alleles associated with adaptation to antibiotics, they do not provide essential information about the relative importance of genomic changes, their order of appearance, or potential epistatic relationships between adaptive changes. Here we used quantitative experimental evolution of a single polymorphic population in continuous culture with whole-genome sequencing and allelic frequency measurements to study daptomycin (DAP) resistance in the vancomycin-resistant clinical pathogen Enterococcus faecalis S613. Importantly, we sustained both planktonic and nonplanktonic (i.e., biofilm) populations in coculture as the concentration of antibiotic was raised, facilitating the development of more ecological complexity than is typically observed in laboratory evolution. Quantitative experimental evolution revealed a clear order and hierarchy of genetic changes leading to resistance, the signaling and metabolic pathways responsible, and the relative importance of these mutations to the evolution of DAP resistance. Despite the relative simplicity of this ex vivo approach compared to the ecological complexity of the human body, we showed that experimental evolution allows for rapid identification of clinically relevant adaptive molecular pathways and new targets for drug design in pathogens.
dc.identifier.citationMiller, Corwin A., Kong, Jiayi, Tran, Truc T., et al.. "Adaptation of Enterococcus faecalis to Daptomycin Reveals an Ordered Progression to Resistance." <i>Antimicrobial Agents and Chemotherapy,</i> 57, no. 11 (2013) American Society for Microbiology: 5373-5383. http://dx.doi.org/10.1128/AAC.01473-13.
dc.identifier.doihttp://dx.doi.org/10.1128/AAC.01473-13
dc.identifier.urihttps://hdl.handle.net/1911/77403
dc.language.isoeng
dc.publisherAmerican Society for Microbiology
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
dc.titleAdaptation of Enterococcus faecalis to Daptomycin Reveals an Ordered Progression to Resistance
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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