Integrating valve-inspired design features into poly(ethylene glycol) hydrogel scaffolds for heart valve tissue engineering

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dc.citation.firstpage11
dc.citation.journalTitleActa Biomaterialia
dc.citation.lastpage21
dc.citation.volumeNumber14
dc.contributor.authorZhang, Xing
dc.contributor.authorXu, Bin
dc.contributor.authorPuperi, Daniel S.
dc.contributor.authorYonezawa, Aline L.
dc.contributor.authorWu, Yan
dc.contributor.authorTseng, Hubert
dc.contributor.authorCuchiara, Maude L.
dc.contributor.authorWest, Jennifer L.
dc.contributor.authorGrande-Allen, K. Jane
dc.date.accessioned2016-08-30T20:50:15Z
dc.date.available2016-08-30T20:50:15Z
dc.date.issued2015
dc.description.abstractThe development of advanced scaffolds that recapitulate the anisotropic mechanical behavior and biological functions of the extracellular matrix in leaflets would be transformative for heart valve tissue engineering. In this study, anisotropic mechanical properties were established in poly(ethylene glycol) (PEG) hydrogels by crosslinking stripes of 3.4 kDa PEG diacrylate (PEGDA) within 20 kDa PEGDA base hydrogels using a photolithographic patterning method. Varying the stripe width and spacing resulted in a tensile elastic modulus parallel to the stripes that was 4.1-6.8 times greater than that in the perpendicular direction, comparable to the degree of anisotropy between the circumferential and radial orientations in native valve leaflets. Biomimetic PEG-peptide hydrogels were prepared by tethering the cell-adhesive peptide RGDS and incorporating the collagenase-degradable peptide PQ (GGGPQG↓IWGQGK) into the polymer network. The specific amounts of RGDS and PEG-PQ within the resulting hydrogels influenced the elongation, de novo extracellular matrix deposition and hydrogel degradation behavior of encapsulated valvular interstitial cells (VICs). In addition, the morphology and activation of VICs grown atop PEG hydrogels could be modulated by controlling the concentration or micro-patterning profile of PEG-RGDS. These results are promising for the fabrication of PEG-based hydrogels using anatomically and biologically inspired scaffold design features for heart valve tissue engineering.
dc.identifier.citationZhang, Xing, Xu, Bin, Puperi, Daniel S., et al.. "Integrating valve-inspired design features into poly(ethylene glycol) hydrogel scaffolds for heart valve tissue engineering." <i>Acta Biomaterialia,</i> 14, (2015) Elsevier: 11-21. http://dx.doi.org/10.1016/j.actbio.2014.11.042.
dc.identifier.doihttp://dx.doi.org/10.1016/j.actbio.2014.11.042
dc.identifier.urihttps://hdl.handle.net/1911/91371
dc.language.isoeng
dc.publisherElsevier
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.
dc.subject.keywordanisotropy
dc.subject.keywordbioactivity
dc.subject.keywordheart valve tissue engineering
dc.subject.keywordhydrogel
dc.subject.keywordPoly(ethylene glycol)
dc.titleIntegrating valve-inspired design features into poly(ethylene glycol) hydrogel scaffolds for heart valve tissue engineering
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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