Integrating valve-inspired design features into poly(ethylene glycol) hydrogel scaffolds for heart valve tissue engineering

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dc.citation.firstpage11en_US
dc.citation.journalTitleActa Biomaterialiaen_US
dc.citation.lastpage21en_US
dc.citation.volumeNumber14en_US
dc.contributor.authorZhang, Xingen_US
dc.contributor.authorXu, Binen_US
dc.contributor.authorPuperi, Daniel S.en_US
dc.contributor.authorYonezawa, Aline L.en_US
dc.contributor.authorWu, Yanen_US
dc.contributor.authorTseng, Huberten_US
dc.contributor.authorCuchiara, Maude L.en_US
dc.contributor.authorWest, Jennifer L.en_US
dc.contributor.authorGrande-Allen, K. Janeen_US
dc.contributor.orgBioengineeringen_US
dc.date.accessioned2016-08-30T20:50:15Zen_US
dc.date.available2016-08-30T20:50:15Zen_US
dc.date.issued2015en_US
dc.description.abstractThe development of advanced scaffolds that recapitulate the anisotropic mechanical behavior and biological functions of the extracellular matrix in leaflets would be transformative for heart valve tissue engineering. In this study, anisotropic mechanical properties were established in poly(ethylene glycol) (PEG) hydrogels by crosslinking stripes of 3.4 kDa PEG diacrylate (PEGDA) within 20 kDa PEGDA base hydrogels using a photolithographic patterning method. Varying the stripe width and spacing resulted in a tensile elastic modulus parallel to the stripes that was 4.1-6.8 times greater than that in the perpendicular direction, comparable to the degree of anisotropy between the circumferential and radial orientations in native valve leaflets. Biomimetic PEG-peptide hydrogels were prepared by tethering the cell-adhesive peptide RGDS and incorporating the collagenase-degradable peptide PQ (GGGPQG↓IWGQGK) into the polymer network. The specific amounts of RGDS and PEG-PQ within the resulting hydrogels influenced the elongation, de novo extracellular matrix deposition and hydrogel degradation behavior of encapsulated valvular interstitial cells (VICs). In addition, the morphology and activation of VICs grown atop PEG hydrogels could be modulated by controlling the concentration or micro-patterning profile of PEG-RGDS. These results are promising for the fabrication of PEG-based hydrogels using anatomically and biologically inspired scaffold design features for heart valve tissue engineering.en_US
dc.identifier.citationZhang, Xing, Xu, Bin, Puperi, Daniel S., et al.. "Integrating valve-inspired design features into poly(ethylene glycol) hydrogel scaffolds for heart valve tissue engineering." <i>Acta Biomaterialia,</i> 14, (2015) Elsevier: 11-21. http://dx.doi.org/10.1016/j.actbio.2014.11.042.en_US
dc.identifier.doihttp://dx.doi.org/10.1016/j.actbio.2014.11.042en_US
dc.identifier.urihttps://hdl.handle.net/1911/91371en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.en_US
dc.subject.keywordanisotropyen_US
dc.subject.keywordbioactivityen_US
dc.subject.keywordheart valve tissue engineeringen_US
dc.subject.keywordhydrogelen_US
dc.subject.keywordPoly(ethylene glycol)en_US
dc.titleIntegrating valve-inspired design features into poly(ethylene glycol) hydrogel scaffolds for heart valve tissue engineeringen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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