Structural characterization of AtmS13, a putative sugar aminotransferase involved in indolocarbazole AT2433 aminopentose biosynthesis

Simple item page
dc.citation.firstpage1547en_US
dc.citation.issueNumber8en_US
dc.citation.journalTitleProteins: Structure, Function, and Bioinformaticsen_US
dc.citation.lastpage1554en_US
dc.citation.volumeNumber83en_US
dc.contributor.authorSingh, Shanterien_US
dc.contributor.authorKim, Youngchangen_US
dc.contributor.authorWang, Fengbinen_US
dc.contributor.authorBigelow, Lanceen_US
dc.contributor.authorEndres, Michaelen_US
dc.contributor.authorKharel, Madan K.en_US
dc.contributor.authorBabnigg, Gyorgyen_US
dc.contributor.authorBingman, Craig A.en_US
dc.contributor.authorJoachimiak, Andrzejen_US
dc.contributor.authorThorson, Jon S.en_US
dc.contributor.authorPhillips, George N.Jr.en_US
dc.date.accessioned2016-10-21T17:01:00Zen_US
dc.date.available2016-10-21T17:01:00Zen_US
dc.date.issued2015en_US
dc.description.abstractAT2433 from Actinomadura melliaura is an indolocarbazole antitumor antibiotic structurally distinguished by its unique aminodideoxypentose-containing disaccharide moiety. The corresponding sugar nucleotide-based biosynthetic pathway for this unusual sugar derives from comparative genomics where AtmS13 has been suggested as the contributing sugar aminotransferase (SAT). Determination of the AtmS13 X-ray structure at 1.50-Å resolution reveals it as a member of the aspartate aminotransferase fold type I (AAT-I). Structural comparisons of AtmS13 with homologous SATs that act upon similar substrates implicate potential active site residues that contribute to distinctions in sugar C5 (hexose vs. pentose) and/or sugar C2 (deoxy vs. hydroxyl) substrate specificity.en_US
dc.identifier.citationSingh, Shanteri, Kim, Youngchang, Wang, Fengbin, et al.. "Structural characterization of AtmS13, a putative sugar aminotransferase involved in indolocarbazole AT2433 aminopentose biosynthesis." <i>Proteins: Structure, Function, and Bioinformatics,</i> 83, no. 8 (2015) Wiley: 1547-1554. http://dx.doi.org/10.1002/prot.24844.en_US
dc.identifier.doihttp://dx.doi.org/10.1002/prot.24844en_US
dc.identifier.urihttps://hdl.handle.net/1911/91979en_US
dc.language.isoengen_US
dc.publisherWileyen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley.en_US
dc.subject.keywordx-ray crystallographyen_US
dc.subject.keywordcarbohydrateen_US
dc.subject.keywordindolocarbazoleen_US
dc.subject.keywordnatural producten_US
dc.subject.keywordsugar nucleotideen_US
dc.subject.keywordtransaminationen_US
dc.titleStructural characterization of AtmS13, a putative sugar aminotransferase involved in indolocarbazole AT2433 aminopentose biosynthesisen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
nihms699679.pdf
Size:
430.8 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Faculty Publications
BioSciences Publications
Chemistry Publications