Structural characterization of AtmS13, a putative sugar aminotransferase involved in indolocarbazole AT2433 aminopentose biosynthesis

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dc.citation.firstpage1547
dc.citation.issueNumber8
dc.citation.journalTitleProteins: Structure, Function, and Bioinformatics
dc.citation.lastpage1554
dc.citation.volumeNumber83
dc.contributor.authorSingh, Shanteri
dc.contributor.authorKim, Youngchang
dc.contributor.authorWang, Fengbin
dc.contributor.authorBigelow, Lance
dc.contributor.authorEndres, Michael
dc.contributor.authorKharel, Madan K.
dc.contributor.authorBabnigg, Gyorgy
dc.contributor.authorBingman, Craig A.
dc.contributor.authorJoachimiak, Andrzej
dc.contributor.authorThorson, Jon S.
dc.contributor.authorPhillips, George N.Jr.
dc.date.accessioned2016-10-21T17:01:00Z
dc.date.available2016-10-21T17:01:00Z
dc.date.issued2015
dc.description.abstractAT2433 from Actinomadura melliaura is an indolocarbazole antitumor antibiotic structurally distinguished by its unique aminodideoxypentose-containing disaccharide moiety. The corresponding sugar nucleotide-based biosynthetic pathway for this unusual sugar derives from comparative genomics where AtmS13 has been suggested as the contributing sugar aminotransferase (SAT). Determination of the AtmS13 X-ray structure at 1.50-Å resolution reveals it as a member of the aspartate aminotransferase fold type I (AAT-I). Structural comparisons of AtmS13 with homologous SATs that act upon similar substrates implicate potential active site residues that contribute to distinctions in sugar C5 (hexose vs. pentose) and/or sugar C2 (deoxy vs. hydroxyl) substrate specificity.
dc.identifier.citationSingh, Shanteri, Kim, Youngchang, Wang, Fengbin, et al.. "Structural characterization of AtmS13, a putative sugar aminotransferase involved in indolocarbazole AT2433 aminopentose biosynthesis." <i>Proteins: Structure, Function, and Bioinformatics,</i> 83, no. 8 (2015) Wiley: 1547-1554. http://dx.doi.org/10.1002/prot.24844.
dc.identifier.doihttp://dx.doi.org/10.1002/prot.24844
dc.identifier.urihttps://hdl.handle.net/1911/91979
dc.language.isoeng
dc.publisherWiley
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley.
dc.subject.keywordx-ray crystallography
dc.subject.keywordcarbohydrate
dc.subject.keywordindolocarbazole
dc.subject.keywordnatural product
dc.subject.keywordsugar nucleotide
dc.subject.keywordtransamination
dc.titleStructural characterization of AtmS13, a putative sugar aminotransferase involved in indolocarbazole AT2433 aminopentose biosynthesis
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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