Optically-sectioned two-shot structured illumination microscopy with Hilbert-Huang processing

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dc.citation.firstpage9517en_US
dc.citation.issueNumber8en_US
dc.citation.journalTitleOptics Expressen_US
dc.citation.lastpage9527en_US
dc.citation.volumeNumber22en_US
dc.contributor.authorPatorski, Krzysztofen_US
dc.contributor.authorTrusiak, Maciejen_US
dc.contributor.authorTkaczyk, Tomaszen_US
dc.contributor.orgBioengineeringen_US
dc.date.accessioned2017-06-05T20:44:20Zen_US
dc.date.available2017-06-05T20:44:20Zen_US
dc.date.issued2014en_US
dc.description.abstractWe introduce a fast, simple, adaptive and experimentally robust method for reconstructing background-rejected optically-sectioned images using two-shot structured illumination microscopy. Our innovative data demodulation method needs two grid-illumination images mutually phase shifted by π (half a grid period) but precise phase displacement between two frames is not required. Upon frames subtraction the input pattern with increased grid modulation is obtained. The first demodulation stage comprises two-dimensional data processing based on the empirical mode decomposition for the object spatial frequency selection (noise reduction and bias term removal). The second stage consists in calculating high contrast image using the two-dimensional spiral Hilbert transform. Our algorithm effectiveness is compared with the results calculated for the same input data using structured-illumination (SIM) and HiLo microscopy methods. The input data were collected for studying highly scattering tissue samples in reflectance mode. Results of our approach compare very favorably with SIM and HiLo techniques.en_US
dc.identifier.citationPatorski, Krzysztof, Trusiak, Maciej and Tkaczyk, Tomasz. "Optically-sectioned two-shot structured illumination microscopy with Hilbert-Huang processing." <i>Optics Express,</i> 22, no. 8 (2014) Optical Society of America: 9517-9527. https://doi.org/10.1364/OE.22.009517.en_US
dc.identifier.doihttps://doi.org/10.1364/OE.22.009517en_US
dc.identifier.urihttps://hdl.handle.net/1911/94797en_US
dc.language.isoengen_US
dc.publisherOptical Society of Americaen_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.titleOptically-sectioned two-shot structured illumination microscopy with Hilbert-Huang processingen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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