Electrically active tissues and cells are found in all kingdoms of life and allow us to perceive, process, and impact our environment. Despite the ubiquity and importance of electrical activity in biology, the genes and proteins controlling many electrically dependent abilities remain incompletely characterized. Electrically excitable cells are highly specialized, frequently resulting in a high degree of heterogeneity, and when analyzing at a population level, the proteins or genes responsible for the behavior of a few cells are quickly drowned out. To facilitate separation of subpopulations of electrically active cells, we have designed, fabricated, and tested a device incorporating nanoelectrodes into a micro uidic chip for sorting cells based on their electrophysiology.