High throughput and rapid isolation of extracellular vesicles and exosomes with purity using size exclusion liquid chromatography

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dc.citation.firstpage683en_US
dc.citation.journalTitleBioactive Materialsen_US
dc.citation.lastpage695en_US
dc.citation.volumeNumber40en_US
dc.contributor.authorKapoor, Kshipra S.en_US
dc.contributor.authorHarris, Kristenen_US
dc.contributor.authorArian, Kent A.en_US
dc.contributor.authorMa, Lihuaen_US
dc.contributor.authorSchueng Zancanela, Beatrizen_US
dc.contributor.authorChurch, Kaira A.en_US
dc.contributor.authorMcAndrews, Kathleen M.en_US
dc.contributor.authorKalluri, Raghuen_US
dc.date.accessioned2024-10-01T14:03:57Zen_US
dc.date.available2024-10-01T14:03:57Zen_US
dc.date.issued2024en_US
dc.description.abstractExtracellular vesicles (EVs) have emerged as potential biomarkers for diagnosing a range of diseases without invasive procedures. Extracellular vesicles also offer advantages compared to synthetic vesicles for delivery of various drugs; however, limitations in segregating EVs from other particles and soluble proteins have led to inconsistent EV retrieval rates with low levels of purity. Here, we report a new high-yield (88.47 %) and rapid (<20 min) EV isolation method termed size exclusion – fast protein liquid chromatography (SE-FPLC). We show SE-FPLC can effectively isolate EVs from multiple sources including EVs derived from human and mouse cells and serum samples. The results indicate that SE-FPLC can successfully remove highly abundant protein contaminants such as albumin and lipoprotein complexes, which can represent a major hurdle in large scale isolation of EVs. The high-yield nature of SE-FPLC allows for easy industrial scaling up of EV production for various clinical utilities. SE-FPLC also enables analysis of small volumes of blood for use in point-of-care diagnostics in the clinic. Collectively, SE-FPLC offers many advantages over current EV isolation methods and offers rapid clinical translation.en_US
dc.identifier.citationKapoor, K. S., Harris, K., Arian, K. A., Ma, L., Schueng Zancanela, B., Church, K. A., McAndrews, K. M., & Kalluri, R. (2024). High throughput and rapid isolation of extracellular vesicles and exosomes with purity using size exclusion liquid chromatography. Bioactive Materials, 40, 683–695. https://doi.org/10.1016/j.bioactmat.2024.08.002en_US
dc.identifier.digital1-s20-S2452199X24003219-mainen_US
dc.identifier.doihttps://doi.org/10.1016/j.bioactmat.2024.08.002en_US
dc.identifier.urihttps://hdl.handle.net/1911/117888en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsExcept where otherwise noted, this work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND) license. Permission to reuse, publish, or reproduce the work beyond the terms of the license or beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.titleHigh throughput and rapid isolation of extracellular vesicles and exosomes with purity using size exclusion liquid chromatographyen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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