Differential structured illumination microendoscopy for in vivo imaging of molecular contrast agents

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dc.citation.firstpage10769
dc.citation.issueNumber9
dc.citation.journalTitleProceedings of the National Academy of Sciences
dc.citation.lastpage10773
dc.citation.volumeNumber113
dc.contributor.authorKeahey, Pelham
dc.contributor.authorRamalingam, Preetha
dc.contributor.authorSchmeler, Kathleen
dc.contributor.authorRichards-Kortum, Rebecca R.
dc.date.accessioned2017-01-09T18:24:39Z
dc.date.available2017-01-09T18:24:39Z
dc.date.issued2016
dc.descriptionNEWS COVERAGE: A news release based on this journal publication is available online: http://news.rice.edu/2016/09/15/improved-microendoscope-brings-cervical-cancer-into-focus/
dc.description.abstractFiber optic microendoscopy has shown promise for visualization of molecular contrast agents used to study disease in vivo. However, fiber optic microendoscopes have limited optical sectioning capability, and image contrast is limited by out-of-focus light generated in highly scattering tissue. Optical sectioning techniques have been used in microendoscopes to remove out-of-focus light but reduce imaging speed or rely on bulky optical elements that prevent in vivo imaging. Here, we present differential structured illumination microendoscopy (DSIMe), a fiber optic system that can perform structured illumination in real time for optical sectioning without any opto-mechanical components attached to the distal tip of the fiber bundle. We demonstrate the use of DSIMe during in vivo fluorescence imaging in patients undergoing surgery for cervical adenocarcinoma in situ. Images acquired using DSIMe show greater contrast than standard microendoscopy, improving the ability to detect cellular atypia associated with neoplasia.
dc.identifier.citationKeahey, Pelham, Ramalingam, Preetha, Schmeler, Kathleen, et al.. "Differential structured illumination microendoscopy for in vivo imaging of molecular contrast agents." <i>Proceedings of the National Academy of Sciences,</i> 113, no. 9 (2016) National Academy of Sciences: 10769-10773. http://dx.doi.org/10.1073/pnas.1613497113.
dc.identifier.doihttp://dx.doi.org/10.1073/pnas.1613497113
dc.identifier.urihttps://hdl.handle.net/1911/93743
dc.language.isoeng
dc.publisherNational Academy of Sciences
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
dc.subject.keywordmolecular imaging
dc.subject.keywordcervical cancer
dc.subject.keywordmicroendoscopy
dc.subject.keywordfluorescence
dc.subject.keywordstructured illumination
dc.titleDifferential structured illumination microendoscopy for in vivo imaging of molecular contrast agents
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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