Unleashing the potential of noncanonical amino acid biosynthesis to create cells with precision tyrosine sulfation

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dc.citation.articleNumber5434en_US
dc.citation.journalTitleNature Communicationsen_US
dc.citation.volumeNumber13en_US
dc.contributor.authorChen, Yudaen_US
dc.contributor.authorJin, Shikaien_US
dc.contributor.authorZhang, Mengxien_US
dc.contributor.authorHu, Yuen_US
dc.contributor.authorWu, Kuan-Linen_US
dc.contributor.authorChung, Annaen_US
dc.contributor.authorWang, Shichaoen_US
dc.contributor.authorTian, Zeruen_US
dc.contributor.authorWang, Yixianen_US
dc.contributor.authorWolynes, Peter G.en_US
dc.contributor.authorXiao, Hanen_US
dc.contributor.orgBioengineeringen_US
dc.contributor.orgBiosciencesen_US
dc.contributor.orgChemistryen_US
dc.contributor.orgPhysics and Astronomyen_US
dc.contributor.orgCenter for Theoretical Biological Physicsen_US
dc.date.accessioned2022-10-28T17:43:10Zen_US
dc.date.available2022-10-28T17:43:10Zen_US
dc.date.issued2022en_US
dc.description.abstractDespite the great promise of genetic code expansion technology to modulate structures and functions of proteins, external addition of ncAAs is required in most cases and it often limits the utility of genetic code expansion technology, especially to noncanonical amino acids (ncAAs) with poor membrane internalization. Here, we report the creation of autonomous cells, both prokaryotic and eukaryotic, with the ability to biosynthesize and genetically encode sulfotyrosine (sTyr), an important protein post-translational modification with low membrane permeability. These engineered cells can produce site-specifically sulfated proteins at a higher yield than cells fed exogenously with the highest level of sTyr reported in the literature. We use these autonomous cells to prepare highly potent thrombin inhibitors with site-specific sulfation. By enhancing ncAA incorporation efficiency, this added ability of cells to biosynthesize ncAAs and genetically incorporate them into proteins greatly extends the utility of genetic code expansion methods.en_US
dc.identifier.citationChen, Yuda, Jin, Shikai, Zhang, Mengxi, et al.. "Unleashing the potential of noncanonical amino acid biosynthesis to create cells with precision tyrosine sulfation." <i>Nature Communications,</i> 13, (2022) Springer Nature: https://doi.org/10.1038/s41467-022-33111-4.en_US
dc.identifier.digitals41467-022-33111-4en_US
dc.identifier.doihttps://doi.org/10.1038/s41467-022-33111-4en_US
dc.identifier.urihttps://hdl.handle.net/1911/113775en_US
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleUnleashing the potential of noncanonical amino acid biosynthesis to create cells with precision tyrosine sulfationen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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