Capture and Release of tRNA by the T-Loop Receptor in the Function of the T-Box Riboswitch

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dc.citation.firstpage3549en_US
dc.citation.issueNumber28en_US
dc.citation.journalTitleBiochemistryen_US
dc.citation.lastpage3558en_US
dc.citation.volumeNumber56en_US
dc.contributor.authorFang, Xianyangen_US
dc.contributor.authorMichnicka, Malgorzataen_US
dc.contributor.authorZhang, Yikanen_US
dc.contributor.authorWang, Yun-Xingen_US
dc.contributor.authorNikonowicz, Edward P.en_US
dc.date.accessioned2017-11-14T18:08:24Zen_US
dc.date.available2017-11-14T18:08:24Zen_US
dc.date.issued2017en_US
dc.description.abstractIn Gram-positive bacteria, the tRNA-dependent T-box riboswitch system regulates expression of amino acid biosynthetic and aminoacyl-tRNA synthetase genes through a transcription attenuation mechanism. Binding of uncharged tRNA “closes” the switch, allowing transcription read-through. Structural studies of the 100-nucleotide stem I domain reveal tRNA utilizes base pairing and stacking interactions to bind the stem, but little is known structurally about the 180-nucleotide riboswitch core (stem I, stem III, and antiterminator stem) in complex with tRNA or the mechanism of coupling of the intermolecular binding domains crucial to T-box function. Here we utilize solution structural and biophysical methods to characterize the interplay of the different riboswitch–tRNA contact points using Bacillus subtilis and Oceanobacillus iheyensis glycyl T-box and T-box:tRNA constructs. The data reveal that tRNA:riboswitch core binding at equilibrium involves only Specifier–anticodon and antiterminator–acceptor stem pairing. The elbow:platform stacking interaction observed in studies of the T-box stem I domain is released after pairing between the acceptor stem and the bulge in the antiterminator helix. The results are consistent with the model of T-box riboswitch:tRNA function in which tRNA is captured by stem I of the nascent mRNA followed by stabilization of the antiterminator helix and the paused transcription complex.en_US
dc.identifier.citationFang, Xianyang, Michnicka, Malgorzata, Zhang, Yikan, et al.. "Capture and Release of tRNA by the T-Loop Receptor in the Function of the T-Box Riboswitch." <i>Biochemistry,</i> 56, no. 28 (2017) American Chemical Society: 3549-3558. https://doi.org/10.1021/acs.biochem.7b00284.en_US
dc.identifier.digitalTbox_SAXS_BCHM_wFIGSen_US
dc.identifier.doihttps://doi.org/10.1021/acs.biochem.7b00284en_US
dc.identifier.urihttps://hdl.handle.net/1911/98807en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the American Chemical Society.en_US
dc.titleCapture and Release of tRNA by the T-Loop Receptor in the Function of the T-Box Riboswitchen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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