Modular probes for enriching and detecting complex nucleic acid sequences

Simple item page
dc.citation.firstpage1222
dc.citation.journalTitleNature Chemistry
dc.citation.lastpage1228
dc.citation.volumeNumber9
dc.contributor.authorWang, Juexiao Sherry
dc.contributor.authorYan, Yan Helen
dc.contributor.authorZhang, David Yu
dc.date.accessioned2019-12-11T15:44:26Z
dc.date.available2019-12-11T15:44:26Z
dc.date.issued2017
dc.description.abstractComplex DNA sequences are difficult to detect and profile, but are important contributors to human health and disease. Existing hybridization probes lack the capability to selectively bind and enrich hypervariable, long or repetitive sequences. Here, we present a generalized strategy for constructing modular hybridization probes (M-Probes) that overcomes these challenges. We demonstrate that M-Probes can tolerate sequence variations of up to 7 nt at prescribed positions while maintaining single nucleotide sensitivity at other positions. M-Probes are also shown to be capable of sequence-selectively binding a continuous DNA sequence of more than 500 nt. Furthermore, we show that M-Probes can detect genes with triplet repeats exceeding a programmed threshold. As a demonstration of this technology, we have developed a hybrid capture method to determine the exact triplet repeat expansion number in the Huntington's gene of genomic DNA using quantitative PCR.
dc.identifier.citationWang, Juexiao Sherry, Yan, Yan Helen and Zhang, David Yu. "Modular probes for enriching and detecting complex nucleic acid sequences." <i>Nature Chemistry,</i> 9, (2017) Springer Nature: 1222-1228. https://doi.org/10.1038/nchem.2820.
dc.identifier.digitalnihms932420
dc.identifier.doihttps://doi.org/10.1038/nchem.2820
dc.identifier.urihttps://hdl.handle.net/1911/107857
dc.language.isoeng
dc.publisherSpringer Nature
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Springer Nature.
dc.titleModular probes for enriching and detecting complex nucleic acid sequences
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
nihms932420.pdf
Size:
1.55 MB
Format:
Adobe Portable Document Format
Download
Collections
Faculty Publications
Bioengineering Publications