Osteochondral Tissue Regeneration using a Bilayered Composite Hydrogel with Modulating Dual Growth Factor Release Kinetics in a Rabbit Model

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dc.citation.firstpage166en_US
dc.citation.issueNumber2en_US
dc.citation.journalTitleJournal of Controlled Releaseen_US
dc.citation.lastpage178en_US
dc.citation.volumeNumber168en_US
dc.contributor.authorKima, Kyobumen_US
dc.contributor.authorLama, Johnnyen_US
dc.contributor.authorLua, Stevenen_US
dc.contributor.authorSpicer, Patrick P.en_US
dc.contributor.authorLueckgena, Alineen_US
dc.contributor.authorYasuhiko, Tabataen_US
dc.contributor.authorWong, Mark E.en_US
dc.contributor.authorJansen, John A.en_US
dc.contributor.authorMikos, Antonios G.en_US
dc.contributor.authorKasper, F. Kurtisen_US
dc.contributor.orgBioengineeringen_US
dc.date.accessioned2014-03-06T18:54:05Zen_US
dc.date.available2014-03-06T18:54:05Zen_US
dc.date.issued2014en_US
dc.description.abstractBiodegradable oligo(poly(ethylene glycol) fumarate) (OPF) composite hydrogels have been investigated for the delivery of growth factors (GFs) with the aid of gelatin microparticles (GMPs) and stem cell populations for osteochondral tissue regeneration. In this study, a bilayered OPF composite hydrogel that mimics the distinctive hierarchical structure of native osteochondral tissue was utilized to investigate the effect of transforming growth factor-β3 (TGF-β3) with varying release kinetics and/or insulin-like growth factor-1 (IGF-1) on osteochondral tissue regeneration in a rabbit full-thickness osteochondral defect model. The four groups investigated included (i) a blank control (no GFs), (ii) GMP-loaded IGF-1 alone, (iii) GMP-loaded IGF-1 and gel-loaded TGF-β3, and (iv) GMP-loaded IGF-1 and GMP-loaded TGF-β3 in OPF composite hydrogels. The results of an in vitro release study demonstrated that TGF-β3 release kinetics could be modulated by the GF incorporation method. At 12 weeks post-implantation, the quality of tissue repair in both chondral and subchondral layers was analyzed based on quantitative histological scoring. All groups incorporating GFs resulted in a significant improvement in cartilage morphology compared to the control. Single delivery of IGF-1 showed higher scores in subchondral bone morphology as well as chondrocyte and glycosaminoglycan amount in adjacent cartilage tissue when compared to a dual delivery of IGF-1 and TGF-β3, independent of the TGF-?3 release kinetics. The results suggest that although the dual delivery of TGF-β3 and IGF-1 may not synergistically enhance the quality of engineered tissue, the delivery of IGF-1 alone from bilayered composite hydrogels positively affects osteochondral tissue repair and holds promise for osteochondral tissue engineering applications.en_US
dc.identifier.citationKima, Kyobum, Lama, Johnny, Lua, Steven, et al.. "Osteochondral Tissue Regeneration using a Bilayered Composite Hydrogel with Modulating Dual Growth Factor Release Kinetics in a Rabbit Model." <i>Journal of Controlled Release,</i> 168, no. 2 (2014) Elsevier: 166-178. http://dx.doi.org/10.1016/j.jconrel.2013.03.013.en_US
dc.identifier.doihttp://dx.doi.org/10.1016/j.jconrel.2013.03.013en_US
dc.identifier.urihttps://hdl.handle.net/1911/75554en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.en_US
dc.subject.keywordhydrogelen_US
dc.subject.keywordosteochondral defecten_US
dc.subject.keywordtransforming growth factor-β3en_US
dc.subject.keywordinsulin-like growth factor-1en_US
dc.titleOsteochondral Tissue Regeneration using a Bilayered Composite Hydrogel with Modulating Dual Growth Factor Release Kinetics in a Rabbit Modelen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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