The use of a microelectroporator to study poration of Jurkat cells

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dc.contributor.advisorRaphael, Robert M.en_US
dc.creatorStark, Daniel Joshua Benedicten_US
dc.date.accessioned2018-12-03T18:33:11Zen_US
dc.date.available2018-12-03T18:33:11Zen_US
dc.date.issued2009en_US
dc.description.abstractA microelectroporation system was built to provide a well-controlled and defined environment for quantitative studies of electroporation, a method to permeabilize cell membranes for delivery of normally impermeable biomolecules, of an immortalized line of T cells. Studying electroporation in a low ionic-strength solution, onset of poration was found to occur at an externally applied field of 700 V/cm, corresponding to a threshold transmembrane potential of 0.83±0.34 V. A finite element model was used to estimate the effect of solution conductivity on the applied electric field, which dominates the uncertainty in this measurement. Furthermore, increased forward light-scattering signal, as measured using flow cytometry, correlated strongly to successful poration. Using forward light-scattering as an indicator identifies a population of cells that is over 90% porated and viable. Quantitative characterization with flow cytometry could serve as a non-invasive assay of microelectroporation in T-cells that can be applied without fluorescent markers.en_US
dc.format.extent94 ppen_US
dc.identifier.callnoTHESIS PHYS. 2010 STARKen_US
dc.identifier.citationStark, Daniel Joshua Benedict. "The use of a microelectroporator to study poration of Jurkat cells." (2009) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/103729">https://hdl.handle.net/1911/103729</a>.en_US
dc.identifier.digital750848665en_US
dc.identifier.urihttps://hdl.handle.net/1911/103729en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectBiomedical engineeringen_US
dc.subjectBiophysicsen_US
dc.subjectApplied sciencesen_US
dc.subjectBiological sciencesen_US
dc.titleThe use of a microelectroporator to study poration of Jurkat cellsen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentPhysicsen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Scienceen_US
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