Dual growth factor delivery from bilayered, biodegradable hydrogel composites for spatially-guided osteochondral tissue repair

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dc.citation.firstpage8829en_US
dc.citation.issueNumber31en_US
dc.citation.journalTitleBiomaterialsen_US
dc.citation.lastpage8839en_US
dc.citation.volumeNumber35en_US
dc.contributor.authorLu, Stevenen_US
dc.contributor.authorLam, Johnnyen_US
dc.contributor.authorTrachtenberg, Jordan E.en_US
dc.contributor.authorLee, Esther J.en_US
dc.contributor.authorSeyednejad, Hajaren_US
dc.contributor.authorvan den Beucken, Jeroen J.J.P.en_US
dc.contributor.authorTabata, Yasuhikoen_US
dc.contributor.authorWong, Mark E.en_US
dc.contributor.authorJansen, John A.en_US
dc.contributor.authorMikos, Antonios G.en_US
dc.contributor.authorKasper, F. Kurtisen_US
dc.contributor.orgBioengineeringen_US
dc.date.accessioned2017-08-04T12:29:41Zen_US
dc.date.available2017-08-04T12:29:41Zen_US
dc.date.issued2014en_US
dc.description.abstractThe present work investigated the use of biodegradable hydrogel composite scaffolds, based on the macromer oligo(poly(ethylene glycol) fumarate) (OPF), to deliver growth factors for the repair of osteochondral tissue in a rabbit model. In particular, bilayered OPF composites were used to mimic the structural layers of the osteochondral unit, and insulin-like growth factor-1 (IGF-1) and bone morphogenetic protein-2 (BMP-2) were loaded into gelatin microparticles and embedded within the OPF hydrogel matrix in a spatially controlled manner. Three different scaffold formulations were implanted in a medial femoral condyle osteochondral defect: 1) IGF-1 in the chondral layer, 2) BMP-2 in the subchondral layer, and 3) IGF-1 and BMP-2 in their respective separate layers. The quantity and quality of osteochondral repair was evaluated at 6 and 12 weeks with histological scoring and micro-computed tomography (micro-CT). While histological scoring results at 6 weeks showed no differences between experimental groups, micro-CT analysis revealed that the delivery of BMP-2 alone increased the number of bony trabecular islets formed, an indication of early bone formation, over that of IGF-1 delivery alone. At 12 weeks post-implantation, minimal differences were detected between the three groups for cartilage repair. However, the dual delivery of IGF-1 and BMP-2 had a higher proportion of subchondral bone repair, greater bone growth at the defect margins, and lower bone specific surface than the single delivery of IGF-1. These results suggest that the delivery of BMP-2 enhances subchondral bone formation and that, while the dual delivery of IGF-1 and BMP-2 in separate layers does not improve cartilage repair under the conditions studied, they may synergistically enhance the degree of subchondral bone formation. Overall, bilayered OPF hydrogel composites demonstrate potential as spatially-guided, multiple growth factor release vehicles for osteochondral tissue repair.en_US
dc.identifier.citationLu, Steven, Lam, Johnny, Trachtenberg, Jordan E., et al.. "Dual growth factor delivery from bilayered, biodegradable hydrogel composites for spatially-guided osteochondral tissue repair." <i>Biomaterials,</i> 35, no. 31 (2014) Elsevier: 8829-8839. https://doi.org/10.1016/j.biomaterials.2014.07.006.en_US
dc.identifier.digitalspatially-guided_osteochondral_tissue_repairen_US
dc.identifier.doihttps://doi.org/10.1016/j.biomaterials.2014.07.006en_US
dc.identifier.urihttps://hdl.handle.net/1911/96578en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.en_US
dc.subject.keywordBone morphogenetic protein-2en_US
dc.subject.keywordCartilage repairen_US
dc.subject.keywordInsulin-like growth factor-1en_US
dc.subject.keywordRabbit modelen_US
dc.subject.keywordSubchondral boneen_US
dc.titleDual growth factor delivery from bilayered, biodegradable hydrogel composites for spatially-guided osteochondral tissue repairen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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