Genetic Suppression of Transgenic APP Rescues Hypersynchronous Network Activity in a Mouse Model of Alzeimer's Disease

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dc.citation.firstpage3826en_US
dc.citation.issueNumber11en_US
dc.citation.journalTitleThe Journal of Neuroscienceen_US
dc.citation.lastpage3840en_US
dc.citation.volumeNumber34en_US
dc.contributor.authorBorn, Heather A.en_US
dc.contributor.authorKim, Ji-Yoenen_US
dc.contributor.authorSavjani, Ricky R.en_US
dc.contributor.authorDas, Pritamen_US
dc.contributor.authorDabaghian, Yuri A.en_US
dc.contributor.authorGuo, Qinxien_US
dc.contributor.authorYoo, Jong W.en_US
dc.contributor.authorSchuler, Dorothy R.en_US
dc.contributor.authorCirrito, John R.en_US
dc.contributor.authorZheng, Huien_US
dc.contributor.authorGolde, Todd E.en_US
dc.contributor.authorNoebels, Jeffrey L.en_US
dc.contributor.authorJankowsky, Joanna L.en_US
dc.date.accessioned2016-02-02T19:17:44Zen_US
dc.date.available2016-02-02T19:17:44Zen_US
dc.date.issued2014en_US
dc.description.abstractAlzheimer's disease (AD) is associated with an elevated risk for seizures that may be fundamentally connected to cognitive dysfunction. Supporting this link, many mouse models for AD exhibit abnormal electroencephalogram (EEG) activity in addition to the expected neuropathology and cognitive deficits. Here, we used a controllable transgenic system to investigate how network changes develop and are maintained in a model characterized by amyloid β (Aβ) overproduction and progressive amyloid pathology. EEG recordings in tet-off mice overexpressing amyloid precursor protein (APP) from birth display frequent sharp wave discharges (SWDs). Unexpectedly, we found that withholding APP overexpression until adulthood substantially delayed the appearance of epileptiform activity. Together, these findings suggest that juvenile APP overexpression altered cortical development to favor synchronized firing. Regardless of the age at which EEG abnormalities appeared, the phenotype was dependent on continued APP overexpression and abated over several weeks once transgene expression was suppressed. Abnormal EEG discharges were independent of plaque load and could be extinguished without altering deposited amyloid. Selective reduction of Aβ with a γ-secretase inhibitor has no effect on the frequency of SWDs, indicating that another APP fragment or the full-length protein was likely responsible for maintaining EEG abnormalities. Moreover, transgene suppression normalized the ratio of excitatory to inhibitory innervation in the cortex, whereas secretase inhibition did not. Our results suggest that APP overexpression, and not Aβ overproduction, is responsible for EEG abnormalities in our transgenic mice and can be rescued independently of pathology.en_US
dc.identifier.citationBorn, Heather A., Kim, Ji-Yoen, Savjani, Ricky R., et al.. "Genetic Suppression of Transgenic APP Rescues Hypersynchronous Network Activity in a Mouse Model of Alzeimer's Disease." <i>The Journal of Neuroscience,</i> 34, no. 11 (2014) Society for Neuroscience: 3826-3840. http://dx.doi.org/10.1523/JNEUROSCI.5171-13.2014.en_US
dc.identifier.doihttp://dx.doi.org/10.1523/JNEUROSCI.5171-13.2014en_US
dc.identifier.urihttps://hdl.handle.net/1911/88304en_US
dc.language.isoengen_US
dc.publisherSociety for Neuroscienceen_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.subject.keywordamyloid precursor proteinen_US
dc.subject.keywordEEGen_US
dc.subject.keywordepilepsyen_US
dc.subject.keywordseizureen_US
dc.subject.keywordsharp wave dischargeen_US
dc.subject.keywordtransgene suppressionen_US
dc.titleGenetic Suppression of Transgenic APP Rescues Hypersynchronous Network Activity in a Mouse Model of Alzeimer's Diseaseen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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