A microfluidic-induced C. elegans sleep state
| dc.citation.articleNumber | 5035 | en_US |
| dc.citation.journalTitle | Nature Communications | en_US |
| dc.citation.volumeNumber | 10 | en_US |
| dc.contributor.author | Gonzales, Daniel L. | en_US |
| dc.contributor.author | Zhou, Jasmine | en_US |
| dc.contributor.author | Fan, Bo | en_US |
| dc.contributor.author | Robinson, Jacob T. | en_US |
| dc.date.accessioned | 2020-02-14T16:39:54Z | en_US |
| dc.date.available | 2020-02-14T16:39:54Z | en_US |
| dc.date.issued | 2019 | en_US |
| dc.description.abstract | An important feature of animal behavior is the ability to switch rapidly between activity states, however, how the brain regulates these spontaneous transitions based on the animal’s perceived environment is not well understood. Here we show a C. elegans sleep-like state on a scalable platform that enables simultaneous control of multiple environmental factors including temperature, mechanical stress, and food availability. This brief quiescent state, which we refer to as microfluidic-induced sleep, occurs spontaneously in microfluidic chambers, which allows us to track animal movement and perform whole-brain imaging. With these capabilities, we establish that microfluidic-induced sleep meets the behavioral requirements of C. elegans sleep and depends on multiple factors, such as satiety and temperature. Additionally, we show that C. elegans sleep can be induced through mechanosensory pathways. Together, these results establish a model system for studying how animals process multiple sensory pathways to regulate behavioral states. | en_US |
| dc.identifier.citation | Gonzales, Daniel L., Zhou, Jasmine, Fan, Bo, et al.. "A microfluidic-induced C. elegans sleep state." <i>Nature Communications,</i> 10, (2019) Springer Nature: https://doi.org/10.1038/s41467-019-13008-5. | en_US |
| dc.identifier.digital | s41467-019-13008-5 | en_US |
| dc.identifier.doi | https://doi.org/10.1038/s41467-019-13008-5 | en_US |
| dc.identifier.uri | https://hdl.handle.net/1911/108055 | en_US |
| dc.language.iso | eng | en_US |
| dc.publisher | Springer Nature | en_US |
| dc.rights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. | en_US |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_US |
| dc.title | A microfluidic-induced C. elegans sleep state | en_US |
| dc.type | Journal article | en_US |
| dc.type.dcmi | Text | en_US |
| dc.type.publication | publisher version | en_US |
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