Disrupting Autophagy Restores Peroxisome Function to an Arabidopsis lon2 Mutant and Reveals a Role for the LON2 Protease in Peroxisomal Matrix Protein Degradation

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dc.citation.firstpage4085
dc.citation.journalTitleThe Plant Cell
dc.citation.lastpage4100
dc.citation.volumeNumber25
dc.contributor.authorFarmer, Lisa M.
dc.contributor.authorRinaldi, Mauro A.
dc.contributor.authorYoung, Pierce G.
dc.contributor.authorDanan, Charles H.
dc.contributor.authorBurkhart, Sarah E.
dc.contributor.authorBartel, Bonnie
dc.date.accessioned2014-01-21T18:41:41Z
dc.date.available2015-01-22T06:10:05Z
dc.date.issued2013
dc.description.abstractPeroxisomes house critical metabolic reactions that are essential for seedling development. As seedlings mature, metabolic requirements change, and peroxisomal contents are remodeled. The resident peroxisomal protease LON2 is positioned to degrade obsolete or damaged peroxisomal proteins, but data supporting such a role in plants have remained elusive. Arabidopsis thaliana lon2 mutants display defects in peroxisomal metabolism and matrix protein import but appear to degrade matrix proteins normally. To elucidate LON2 functions, we executed a forward-genetic screen for lon2 suppressors, which revealed multiple mutations in key autophagy genes. Disabling core autophagy-related gene (ATG) products prevents autophagy, a process through which cytosolic constituents, including organelles, can be targeted for vacuolar degradation. We found that atg2, atg3, and atg7 mutations suppressed lon2 defects in auxin metabolism and matrix protein processing and rescued the abnormally large size and small number of lon2 peroxisomes. Moreover, analysis of lon2 atg mutants uncovered an apparent role for LON2 in matrix protein turnover. Our data suggest that LON2 facilitates matrix protein degradation during peroxisome content remodeling, provide evidence for the existence of pexophagy in plants, and indicate that peroxisome destruction via autophagy is enhanced when LON2 is absent.
dc.embargo.terms1 year
dc.identifier.citationFarmer, Lisa M., Rinaldi, Mauro A., Young, Pierce G., et al.. "Disrupting Autophagy Restores Peroxisome Function to an Arabidopsis lon2 Mutant and Reveals a Role for the LON2 Protease in Peroxisomal Matrix Protein Degradation." <i>The Plant Cell,</i> 25, (2013) American Society of Plant Biologists: 4085-4100. http://dx.doi.org/10.1105/tpc.113.113407.
dc.identifier.doihttp://dx.doi.org/10.1105/tpc.113.113407
dc.identifier.urihttps://hdl.handle.net/1911/75341
dc.language.isoeng
dc.publisherAmerican Society of Plant Biologists
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
dc.titleDisrupting Autophagy Restores Peroxisome Function to an Arabidopsis lon2 Mutant and Reveals a Role for the LON2 Protease in Peroxisomal Matrix Protein Degradation
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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