Disrupting Autophagy Restores Peroxisome Function to an Arabidopsis lon2 Mutant and Reveals a Role for the LON2 Protease in Peroxisomal Matrix Protein Degradation

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dc.citation.firstpage4085en_US
dc.citation.journalTitleThe Plant Cellen_US
dc.citation.lastpage4100en_US
dc.citation.volumeNumber25en_US
dc.contributor.authorFarmer, Lisa M.en_US
dc.contributor.authorRinaldi, Mauro A.en_US
dc.contributor.authorYoung, Pierce G.en_US
dc.contributor.authorDanan, Charles H.en_US
dc.contributor.authorBurkhart, Sarah E.en_US
dc.contributor.authorBartel, Bonnieen_US
dc.date.accessioned2014-01-21T18:41:41Zen_US
dc.date.available2015-01-22T06:10:05Zen_US
dc.date.issued2013en_US
dc.description.abstractPeroxisomes house critical metabolic reactions that are essential for seedling development. As seedlings mature, metabolic requirements change, and peroxisomal contents are remodeled. The resident peroxisomal protease LON2 is positioned to degrade obsolete or damaged peroxisomal proteins, but data supporting such a role in plants have remained elusive. Arabidopsis thaliana lon2 mutants display defects in peroxisomal metabolism and matrix protein import but appear to degrade matrix proteins normally. To elucidate LON2 functions, we executed a forward-genetic screen for lon2 suppressors, which revealed multiple mutations in key autophagy genes. Disabling core autophagy-related gene (ATG) products prevents autophagy, a process through which cytosolic constituents, including organelles, can be targeted for vacuolar degradation. We found that atg2, atg3, and atg7 mutations suppressed lon2 defects in auxin metabolism and matrix protein processing and rescued the abnormally large size and small number of lon2 peroxisomes. Moreover, analysis of lon2 atg mutants uncovered an apparent role for LON2 in matrix protein turnover. Our data suggest that LON2 facilitates matrix protein degradation during peroxisome content remodeling, provide evidence for the existence of pexophagy in plants, and indicate that peroxisome destruction via autophagy is enhanced when LON2 is absent.en_US
dc.embargo.terms1 yearen_US
dc.identifier.citationFarmer, Lisa M., Rinaldi, Mauro A., Young, Pierce G., et al.. "Disrupting Autophagy Restores Peroxisome Function to an Arabidopsis lon2 Mutant and Reveals a Role for the LON2 Protease in Peroxisomal Matrix Protein Degradation." <i>The Plant Cell,</i> 25, (2013) American Society of Plant Biologists: 4085-4100. http://dx.doi.org/10.1105/tpc.113.113407.en_US
dc.identifier.doihttp://dx.doi.org/10.1105/tpc.113.113407en_US
dc.identifier.urihttps://hdl.handle.net/1911/75341en_US
dc.language.isoengen_US
dc.publisherAmerican Society of Plant Biologistsen_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.titleDisrupting Autophagy Restores Peroxisome Function to an Arabidopsis lon2 Mutant and Reveals a Role for the LON2 Protease in Peroxisomal Matrix Protein Degradationen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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