Environmental DNA (eDNA) detects the invasive rusty crayfish Orconectes rusticus at low abundances

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dc.citation.firstpage722
dc.citation.journalTitleJournal of Applied Ecology
dc.citation.lastpage732
dc.citation.volumeNumber53
dc.contributor.authorDougherty, Matthew M.
dc.contributor.authorLarson, Eric R.
dc.contributor.authorRenshaw, Mark A.
dc.contributor.authorGantz, Crysta A.
dc.contributor.authorEgan, Scott P.
dc.contributor.authorErickson, Daniel M.
dc.contributor.authorLodge, David M.
dc.date.accessioned2017-05-15T21:11:38Z
dc.date.available2017-05-15T21:11:38Z
dc.date.issued2016
dc.description.abstract1. Early detection is invaluable for the cost-effective control and eradication of invasive species, yet many traditional sampling techniques are ineffective at the low population abundances found at the onset of the invasion process. Environmental DNA (eDNA) is a promising and sensitive tool for early detection of some invasive species, but its efficacy has not yet been evaluated for many taxonomic groups and habitat types. 2. We evaluated the ability of eDNA to detect the invasive rusty crayfish Orconectes rusticus and to reflect patterns of its relative abundance, in upper Midwest, USA, inland lakes. We paired conventional baited trapping as a measure of crayfish relative abundance with water samples for eDNA, which were analysed in the laboratory with a qPCR assay. We modelled detection probability for O. rusticus eDNA using relative abundance and site characteristics as covariates and also tested the relationship between eDNA copy number and O. rusticus relative abundance. 3. We detected O. rusticus eDNA in all lakes where this species was collected by trapping, down to low relative abundances, as well as in two lakes where trap catch was zero. Detection probability of O. rusticus eDNA was well predicted by relative abundance of this species and lake water clarity. However, there was poor correspondence between eDNA copy number and O. rusticus relative abundance estimated by trap catches. 4. Synthesis and applications. Our study demonstrates a field and laboratory protocol for eDNA monitoring of crayfish invasions, with results of statistical models that provide guidance of sampling effort and detection probabilities for researchers in other regions and systems. We propose eDNA be included as a tool in surveillance for invasive or imperiled crayfishes and other benthic arthropods.
dc.identifier.citationDougherty, Matthew M., Larson, Eric R., Renshaw, Mark A., et al.. "Environmental DNA (eDNA) detects the invasive rusty crayfish Orconectes rusticus at low abundances." <i>Journal of Applied Ecology,</i> 53, (2016) Wiley: 722-732. https://doi.org/10.1111/1365-2664.12621.
dc.identifier.doihttps://doi.org/10.1111/1365-2664.12621
dc.identifier.urihttps://hdl.handle.net/1911/94274
dc.language.isoeng
dc.publisherWiley
dc.rightsThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.keywordcrayfish
dc.subject.keyworddetection probability
dc.subject.keywordearly detection
dc.subject.keywordearly warning
dc.subject.keywordexotic species
dc.subject.keywordinvasive species
dc.subject.keywordlake
dc.subject.keywordnon‐indigenous
dc.subject.keywordoccupancy estimation
dc.subject.keywordquantitative PCR (qPCR)
dc.titleEnvironmental DNA (eDNA) detects the invasive rusty crayfish Orconectes rusticus at low abundances
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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