Environmental DNA (eDNA) detects the invasive rusty crayfish Orconectes rusticus at low abundances

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dc.citation.firstpage722en_US
dc.citation.journalTitleJournal of Applied Ecologyen_US
dc.citation.lastpage732en_US
dc.citation.volumeNumber53en_US
dc.contributor.authorDougherty, Matthew M.en_US
dc.contributor.authorLarson, Eric R.en_US
dc.contributor.authorRenshaw, Mark A.en_US
dc.contributor.authorGantz, Crysta A.en_US
dc.contributor.authorEgan, Scott P.en_US
dc.contributor.authorErickson, Daniel M.en_US
dc.contributor.authorLodge, David M.en_US
dc.date.accessioned2017-05-15T21:11:38Zen_US
dc.date.available2017-05-15T21:11:38Zen_US
dc.date.issued2016en_US
dc.description.abstract1. Early detection is invaluable for the cost-effective control and eradication of invasive species, yet many traditional sampling techniques are ineffective at the low population abundances found at the onset of the invasion process. Environmental DNA (eDNA) is a promising and sensitive tool for early detection of some invasive species, but its efficacy has not yet been evaluated for many taxonomic groups and habitat types. 2. We evaluated the ability of eDNA to detect the invasive rusty crayfish Orconectes rusticus and to reflect patterns of its relative abundance, in upper Midwest, USA, inland lakes. We paired conventional baited trapping as a measure of crayfish relative abundance with water samples for eDNA, which were analysed in the laboratory with a qPCR assay. We modelled detection probability for O. rusticus eDNA using relative abundance and site characteristics as covariates and also tested the relationship between eDNA copy number and O. rusticus relative abundance. 3. We detected O. rusticus eDNA in all lakes where this species was collected by trapping, down to low relative abundances, as well as in two lakes where trap catch was zero. Detection probability of O. rusticus eDNA was well predicted by relative abundance of this species and lake water clarity. However, there was poor correspondence between eDNA copy number and O. rusticus relative abundance estimated by trap catches. 4. Synthesis and applications. Our study demonstrates a field and laboratory protocol for eDNA monitoring of crayfish invasions, with results of statistical models that provide guidance of sampling effort and detection probabilities for researchers in other regions and systems. We propose eDNA be included as a tool in surveillance for invasive or imperiled crayfishes and other benthic arthropods.en_US
dc.identifier.citationDougherty, Matthew M., Larson, Eric R., Renshaw, Mark A., et al.. "Environmental DNA (eDNA) detects the invasive rusty crayfish Orconectes rusticus at low abundances." <i>Journal of Applied Ecology,</i> 53, (2016) Wiley: 722-732. https://doi.org/10.1111/1365-2664.12621.en_US
dc.identifier.doihttps://doi.org/10.1111/1365-2664.12621en_US
dc.identifier.urihttps://hdl.handle.net/1911/94274en_US
dc.language.isoengen_US
dc.publisherWileyen_US
dc.rightsThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subject.keywordcrayfishen_US
dc.subject.keyworddetection probabilityen_US
dc.subject.keywordearly detectionen_US
dc.subject.keywordearly warningen_US
dc.subject.keywordexotic speciesen_US
dc.subject.keywordinvasive speciesen_US
dc.subject.keywordlakeen_US
dc.subject.keywordnon‐indigenousen_US
dc.subject.keywordoccupancy estimationen_US
dc.subject.keywordquantitative PCR (qPCR)en_US
dc.titleEnvironmental DNA (eDNA) detects the invasive rusty crayfish Orconectes rusticus at low abundancesen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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