Single-molecule force measurements of the polymerizing dimeric subunit of von Willebrand factor

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dc.citation.firstpage12410en_US
dc.citation.issueNumber1en_US
dc.citation.journalTitlePhysical Review Een_US
dc.citation.volumeNumber93en_US
dc.contributor.authorWijeratne, Sithara S.en_US
dc.contributor.authorLi, Jingqiangen_US
dc.contributor.authorYeh, Hui-Chunen_US
dc.contributor.authorNolasco, Leticiaen_US
dc.contributor.authorZhou, Zhouen_US
dc.contributor.authorBergeron, Angelaen_US
dc.contributor.authorFrey, Eric W.en_US
dc.contributor.authorMoake, Joel L.en_US
dc.contributor.authorDong, Jing-feien_US
dc.contributor.authorKiang, Ching-Hwaen_US
dc.date.accessioned2016-05-02T16:21:05Zen_US
dc.date.available2016-05-02T16:21:05Zen_US
dc.date.issued2016en_US
dc.description.abstractVon Willebrand factor (VWF) multimers are large adhesive proteins that are essential to the initiation of hemostatic plugs at sites of vascular injury. The binding of VWF multimers to platelets, as well as VWF proteolysis, is regulated by shear stresses that alter VWF multimeric conformation. We used single molecule manipulation with atomic force microscopy (AFM) to investigate the effect of high fluid shear stress on soluble dimeric and multimeric forms of VWF. VWF dimers are the smallest unit that polymerizes to construct large VWF multimers. The resistance to mechanical unfolding with or without exposure to shear stress was used to evaluate VWF conformational forms. Our data indicate that, unlike recombinant VWF multimers (RVWF), recombinant dimeric VWF (RDVWF) unfolding force is not altered by high shear stress (100dynes/cm2 for 3 min at 37∘C). We conclude that under the shear conditions used (100dynes/cm2 for 3 min at 37∘C), VWF dimers do not self-associate into a conformation analogous to that attained by sheared large VWF multimers.en_US
dc.identifier.citationWijeratne, Sithara S., Li, Jingqiang, Yeh, Hui-Chun, et al.. "Single-molecule force measurements of the polymerizing dimeric subunit of von Willebrand factor." <i>Physical Review E,</i> 93, no. 1 (2016) American Physical Society: 012410. http://dx.doi.org/10.1103/PhysRevE.93.012410.en_US
dc.identifier.doihttp://dx.doi.org/10.1103/PhysRevE.93.012410en_US
dc.identifier.urihttps://hdl.handle.net/1911/90387en_US
dc.language.isoengen_US
dc.publisherAmerican Physical Societyen_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.titleSingle-molecule force measurements of the polymerizing dimeric subunit of von Willebrand factoren_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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