Rapid Molecular Detection of Invasive Species in Ballast and Harbor Water by Integrating Environmental DNA and Light Transmission Spectroscopy

Simple item page
dc.citation.firstpage4113
dc.citation.issueNumber7
dc.citation.journalTitleEnvironmental Science & Technology
dc.citation.lastpage4121
dc.citation.volumeNumber49
dc.contributor.authorEgan, Scott P.
dc.contributor.authorGrey, Erin K.
dc.contributor.authorOlds, Brett
dc.contributor.authorFeder, Jeffrey L.
dc.contributor.authorRuggiero, Steven T.
dc.contributor.authorTanner, Carol E.
dc.contributor.authorLodge, David M.
dc.date.accessioned2016-01-29T22:36:34Z
dc.date.available2016-01-29T22:36:34Z
dc.date.issued2015
dc.description.abstractInvasive species introduced via the ballast water of commercial ships cause enormous environmental and economic damage worldwide. Accurate monitoring for these often microscopic and morphologically indistinguishable species is challenging but critical for mitigating damages. We apply eDNA sampling, which involves the filtering and subsequent DNA extraction of microscopic bits of tissue suspended in water, to ballast and harbor water sampled during a commercial shipﾒs 1400 km voyage through the North American Great Lakes. Using a lab-based gel electrophoresis assay and a rapid, field-ready light transmission spectroscopy (LTS) assay, we test for the presence of two invasive species: quagga (Dreissena bugensis) and zebra (D. polymorpha) mussels. Furthermore, we spiked a set of uninfested ballast and harbor samples with zebra mussel tissue to further test each assayﾒs detection capabilities. In unmanipulated samples, zebra mussel was not detected, while quagga mussel was detected in all samples at a rate of 85% for the gel assay and 100% for the LTS assay. In the spiked experimental samples, both assays detected zebra mussel in 94% of spiked samples and 0% of negative controls. Overall, these results demonstrate that eDNA sampling is effective for monitoring ballast-mediated invasions and that LTS has the potential for rapid, field-based detection.
dc.identifier.citationEgan, Scott P., Grey, Erin K., Olds, Brett, et al.. "Rapid Molecular Detection of Invasive Species in Ballast and Harbor Water by Integrating Environmental DNA and Light Transmission Spectroscopy." <i>Environmental Science & Technology,</i> 49, no. 7 (2015) American Chemical Society: 4113-4121. http://dx.doi.org/10.1021/es5058659.
dc.identifier.doihttp://dx.doi.org/10.1021/es5058659
dc.identifier.urihttps://hdl.handle.net/1911/88291
dc.language.isoeng
dc.publisherAmerican Chemical Society
dc.rightsThis is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
dc.rights.urihttp://pubs.acs.org/page/policy/authorchoice_termsofuse.html
dc.titleRapid Molecular Detection of Invasive Species in Ballast and Harbor Water by Integrating Environmental DNA and Light Transmission Spectroscopy
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
es5058659.pdf
Size:
948.65 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Faculty Publications
BioSciences Publications