Light Sheet Illumination for 3D Single-Molecule Super-Resolution Imaging of Neuronal Synapses

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dc.citation.articleNumber761530en_US
dc.citation.journalTitleFrontiers in Synaptic Neuroscienceen_US
dc.citation.volumeNumber13en_US
dc.contributor.authorGagliano, Gabriellaen_US
dc.contributor.authorNelson, Tyleren_US
dc.contributor.authorSaliba, Nahimaen_US
dc.contributor.authorVargas-Hernández, Sofíaen_US
dc.contributor.authorGustavsson, Anna-Karinen_US
dc.contributor.orgSmalley-Curl Instituteen_US
dc.contributor.orgLaboratory for Nanophotonicsen_US
dc.contributor.orgInstitute of Biosciences & Bioengineeringen_US
dc.date.accessioned2022-01-07T16:17:21Zen_US
dc.date.available2022-01-07T16:17:21Zen_US
dc.date.issued2021en_US
dc.description.abstractThe function of the neuronal synapse depends on the dynamics and interactions of individual molecules at the nanoscale. With the development of single-molecule super-resolution microscopy over the last decades, researchers now have a powerful and versatile imaging tool for mapping the molecular mechanisms behind the biological function. However, imaging of thicker samples, such as mammalian cells and tissue, in all three dimensions is still challenging due to increased fluorescence background and imaging volumes. The combination of single-molecule imaging with light sheet illumination is an emerging approach that allows for imaging of biological samples with reduced fluorescence background, photobleaching, and photodamage. In this review, we first present a brief overview of light sheet illumination and previous super-resolution techniques used for imaging of neurons and synapses. We then provide an in-depth technical review of the fundamental concepts and the current state of the art in the fields of three-dimensional single-molecule tracking and super-resolution imaging with light sheet illumination. We review how light sheet illumination can improve single-molecule tracking and super-resolution imaging in individual neurons and synapses, and we discuss emerging perspectives and new innovations that have the potential to enable and improve single-molecule imaging in brain tissue.en_US
dc.identifier.citationGagliano, Gabriella, Nelson, Tyler, Saliba, Nahima, et al.. "Light Sheet Illumination for 3D Single-Molecule Super-Resolution Imaging of Neuronal Synapses." <i>Frontiers in Synaptic Neuroscience,</i> 13, (2021) Frontiers Media S.A.: https://doi.org/10.3389/fnsyn.2021.761530.en_US
dc.identifier.digitalfnsyn-13-761530en_US
dc.identifier.doihttps://doi.org/10.3389/fnsyn.2021.761530en_US
dc.identifier.urihttps://hdl.handle.net/1911/111916en_US
dc.language.isoengen_US
dc.publisherFrontiers Media S.A.en_US
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleLight Sheet Illumination for 3D Single-Molecule Super-Resolution Imaging of Neuronal Synapsesen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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