Harshly Oxidized Activated Charcoal Enhances Protein Persulfidation with Implications for Neurodegeneration as Exemplified by Friedreich’s Ataxia

Simple item page
dc.citation.articleNumber2007en_US
dc.citation.issueNumber24en_US
dc.citation.journalTitleNanomaterialsen_US
dc.citation.volumeNumber14en_US
dc.contributor.authorVo, Anh T. T.en_US
dc.contributor.authorKhan, Uffafen_US
dc.contributor.authorLiopo, Anton V.en_US
dc.contributor.authorMouli, Karthiken_US
dc.contributor.authorOlson, Kenneth R.en_US
dc.contributor.authorMcHugh, Emily A.en_US
dc.contributor.authorTour, James M.en_US
dc.contributor.authorPooparayil Manoj, Madhavanen_US
dc.contributor.authorDerry, Paul J.en_US
dc.contributor.authorKent, Thomas A.en_US
dc.contributor.orgSmalley-Curl Institute;Rice Advanced Materials Institute;The NanoCarbon Centeren_US
dc.date.accessioned2025-01-09T20:17:02Zen_US
dc.date.available2025-01-09T20:17:02Zen_US
dc.date.issued2024en_US
dc.description.abstractHarsh acid oxidation of activated charcoal transforms an insoluble carbon-rich source into water-soluble, disc structures of graphene decorated with multiple oxygen-containing functionalities. We term these pleiotropic nano-enzymes as “pleozymes”. A broad redox potential spans many crucial redox reactions including the oxidation of hydrogen sulfide (H2S) to polysulfides and thiosulfate, dismutation of the superoxide radical (O2−*), and oxidation of NADH to NAD+. The oxidation of H2S is predicted to enhance protein persulfidation—the attachment of sulfur to cysteine residues. Persulfidated proteins act as redox intermediates, and persulfidation protects proteins from irreversible oxidation and ubiquitination, providing an important means of signaling. Protein persulfidation is believed to decline in several neurological disorders and aging. Importantly, and consistent with the role of persulfidation in signaling, the master antioxidant transcription factor Nrf2 is regulated by Keap1’s persulfidation. Here, we demonstrate that pleozymes increased overall protein persulfidation in cells from apparently healthy individuals and from individuals with the mitochondrial protein mutation responsible for Friedreich’s ataxia. We further find that pleozymes specifically enhanced Keap1 persulfidation, with subsequent increased accumulation of Nrf2 and Nrf2’s antioxidant targets.en_US
dc.identifier.citationVo, A. T. T., Khan, U., Liopo, A. V., Mouli, K., Olson, K. R., McHugh, E. A., Tour, J. M., Pooparayil Manoj, M., Derry, P. J., & Kent, T. A. (2024). Harshly Oxidized Activated Charcoal Enhances Protein Persulfidation with Implications for Neurodegeneration as Exemplified by Friedreich’s Ataxia. Nanomaterials, 14(24), Article 24. https://doi.org/10.3390/nano14242007en_US
dc.identifier.digitalnanomaterials-14-02007-v3en_US
dc.identifier.doihttps://doi.org/10.3390/nano14242007en_US
dc.identifier.urihttps://hdl.handle.net/1911/118135en_US
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.rightsExcept where otherwise noted, this work is licensed under a Creative Commons Attribution (CC BY) license. Permission to reuse, publish, or reproduce the work beyond the terms of the license or beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subject.keywordnanozymeen_US
dc.subject.keywordpersulfidationen_US
dc.subject.keywordNrf2en_US
dc.subject.keywordKeap1en_US
dc.subject.keywordoxidized activated carbon nanoparticlesen_US
dc.titleHarshly Oxidized Activated Charcoal Enhances Protein Persulfidation with Implications for Neurodegeneration as Exemplified by Friedreich’s Ataxiaen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
nanomaterials-14-02007-v3.pdf
Size:
2.11 MB
Format:
Adobe Portable Document Format
Download
Collections
Faculty Publications