Structural Basis for the Stereochemical Control of Amine Installation in Nucleotide Sugar Aminotransferases

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dc.citation.firstpage2048en_US
dc.citation.issueNumber9en_US
dc.citation.journalTitleACS Chemical Biologyen_US
dc.citation.lastpage2056en_US
dc.citation.volumeNumber10en_US
dc.contributor.authorWang, Fengbinen_US
dc.contributor.authorSingh, Shanterien_US
dc.contributor.authorXu, Weijunen_US
dc.contributor.authorHelmich, Kate E.en_US
dc.contributor.authorMiller, Mitchell D.en_US
dc.contributor.authorCao, Hongnanen_US
dc.contributor.authorBingman, Craig A.en_US
dc.contributor.authorThorson, Jon S.en_US
dc.contributor.authorPhillips, George N.Jr.en_US
dc.date.accessioned2016-10-21T17:01:00Zen_US
dc.date.available2016-10-21T17:01:00Zen_US
dc.date.issued2015en_US
dc.description.abstractSugar aminotransferases (SATs) are an important class of tailoring enzymes that catalyze the 5'-pyridoxal phosphate (PLP)-dependent stereo- and regiospecific installation of an amino group from an amino acid donor (typically L-Glu or L-Gln) to a corresponding ketosugar nucleotide acceptor. Herein we report the strategic structural study of two homologous C4 SATs (Micromonospora echinospora CalS13 and Escherichia coli WecE) that utilize identical substrates but differ in their stereochemistry of aminotransfer. This study reveals for the first time a new mode of SAT sugar nucleotide binding and, in conjunction with previously reported SAT structural studies, provides the basis from which to propose a universal model for SAT stereo- and regiochemical control of amine installation. Specifically, the universal model put forth highlights catalytic divergence to derive solely from distinctions within nucleotide sugar orientation upon binding within a relatively fixed SAT active site where the available ligand bound structures of the three out of four representative C3 and C4 SAT examples provide a basis for the overall model. Importantly, this study presents a new predictive model to support SAT functional annotation, biochemical study and rational engineering.en_US
dc.identifier.citationWang, Fengbin, Singh, Shanteri, Xu, Weijun, et al.. "Structural Basis for the Stereochemical Control of Amine Installation in Nucleotide Sugar Aminotransferases." <i>ACS Chemical Biology,</i> 10, no. 9 (2015) American Chemical Society: 2048-2056. http://dx.doi.org/10.1021/acschembio.5b00244.en_US
dc.identifier.doihttp://dx.doi.org/10.1021/acschembio.5b00244en_US
dc.identifier.urihttps://hdl.handle.net/1911/91978en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the American Chemical Society.en_US
dc.titleStructural Basis for the Stereochemical Control of Amine Installation in Nucleotide Sugar Aminotransferasesen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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