Intratumoral Heterogeneity and Clonal Evolution Induced by HPV Integration

Simple item page
dc.citation.firstpage910
dc.citation.issueNumber4
dc.citation.journalTitleCancer Discovery
dc.citation.lastpage927
dc.citation.volumeNumber13
dc.contributor.authorAkagi, Keiko
dc.contributor.authorSymer, David E.
dc.contributor.authorMahmoud, Medhat
dc.contributor.authorJiang, Bo
dc.contributor.authorGoodwin, Sara
dc.contributor.authorWangsa, Darawalee
dc.contributor.authorLi, Zhengke
dc.contributor.authorXiao, Weihong
dc.contributor.authorDan Dunn, Joe
dc.contributor.authorRied, Thomas
dc.contributor.authorCoombes, Kevin R.
dc.contributor.authorSedlazeck, Fritz J.
dc.contributor.authorGillison, Maura L.
dc.date.accessioned2023-04-25T14:47:45Z
dc.date.available2023-04-25T14:47:45Z
dc.date.issued2023
dc.description.abstractThe human papillomavirus (HPV) genome is integrated into host DNA in most HPV-positive cancers, but the consequences for chromosomal integrity are unknown. Continuous long-read sequencing of oropharyngeal cancers and cancer cell lines identified a previously undescribed form of structural variation, “heterocateny,” characterized by diverse, interrelated, and repetitive patterns of concatemerized virus and host DNA segments within a cancer. Unique breakpoints shared across structural variants facilitated stepwise reconstruction of their evolution from a common molecular ancestor. This analysis revealed that virus and virus–host concatemers are unstable and, upon insertion into and excision from chromosomes, facilitate capture, amplification, and recombination of host DNA and chromosomal rearrangements. Evidence of heterocateny was detected in extrachromosomal and intrachromosomal DNA. These findings indicate that heterocateny is driven by the dynamic, aberrant replication and recombination of an oncogenic DNA virus, thereby extending known consequences of HPV integration to include promotion of intratumoral heterogeneity and clonal evolution.Long-read sequencing of HPV-positive cancers revealed “heterocateny,” a previously unreported form of genomic structural variation characterized by heterogeneous, interrelated, and repetitive genomic rearrangements within a tumor. Heterocateny is driven by unstable concatemerized HPV genomes, which facilitate capture, rearrangement, and amplification of host DNA, and promotes intratumoral heterogeneity and clonal evolution.See related commentary by McBride and White, p. 814.This article is highlighted in the In This Issue feature, p. 799
dc.identifier.citationAkagi, Keiko, Symer, David E., Mahmoud, Medhat, et al.. "Intratumoral Heterogeneity and Clonal Evolution Induced by HPV Integration." <i>Cancer Discovery,</i> 13, no. 4 (2023) AACR: 910-927. https://doi.org/10.1158/2159-8290.CD-22-0900.
dc.identifier.digital910
dc.identifier.doihttps://doi.org/10.1158/2159-8290.CD-22-0900
dc.identifier.urihttps://hdl.handle.net/1911/114817
dc.language.isoeng
dc.publisherAACR
dc.rightsThis open access article is distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) license.
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleIntratumoral Heterogeneity and Clonal Evolution Induced by HPV Integration
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
910.pdf
Size:
9.05 MB
Format:
Adobe Portable Document Format
Download
Collections
Faculty Publications
Computer Science Publications