Native characterization of nucleic acid motif thermodynamics via non-covalent catalysis

Simple item page
dc.citation.articleNumber10319en_US
dc.citation.journalTitleNature Communicationsen_US
dc.citation.volumeNumber7en_US
dc.contributor.authorWang, Chunyanen_US
dc.contributor.authorBae, Jin H.en_US
dc.contributor.authorZhang, David Yuen_US
dc.contributor.orgBioengineeringen_US
dc.contributor.orgSystems, Synthetic, and Physical Biologyen_US
dc.date.accessioned2016-06-06T16:48:22Zen_US
dc.date.available2016-06-06T16:48:22Zen_US
dc.date.issued2016en_US
dc.description.abstractDNA hybridization thermodynamics is critical for accurate design of oligonucleotides for biotechnology and nanotechnology applications, but parameters currently in use are inaccurately extrapolated based on limited quantitative understanding of thermal behaviours. Here, we present a method to measure the ΔG° of DNA motifs at temperatures and buffer conditions of interest, with significantly better accuracy (6- to 14-fold lower s.e.) than prior methods. The equilibrium constant of a reaction with thermodynamics closely approximating that of a desired motif is numerically calculated from directly observed reactant and product equilibrium concentrations; a DNA catalyst is designed to accelerate equilibration. We measured the ΔG° of terminal fluorophores, single-nucleotide dangles and multinucleotide dangles, in temperatures ranging from 10 to 45 °C.en_US
dc.identifier.citationWang, Chunyan, Bae, Jin H. and Zhang, David Yu. "Native characterization of nucleic acid motif thermodynamics via non-covalent catalysis." <i>Nature Communications,</i> 7, (2016) Nature Publishing Group: http://dx.doi.org/10.1038/ncomms10319.en_US
dc.identifier.doihttp://dx.doi.org/10.1038/ncomms10319en_US
dc.identifier.urihttps://hdl.handle.net/1911/90448en_US
dc.language.isoengen_US
dc.publisherNature Publishing Groupen_US
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the articleﾒs Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.titleNative characterization of nucleic acid motif thermodynamics via non-covalent catalysisen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ncomms10319.pdf
Size:
849.55 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Faculty Publications