Ultrasensitive Plasmonic Platform for Label-Free Detection of Membrane-Associated Species

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dc.citation.firstpage7968en_US
dc.citation.issueNumber16en_US
dc.citation.journalTitleAnalytical Chemistryen_US
dc.citation.lastpage7974en_US
dc.citation.volumeNumber88en_US
dc.contributor.authorBruzas, Ianen_US
dc.contributor.authorUnser, Sarahen_US
dc.contributor.authorYazdi, Sadeghen_US
dc.contributor.authorRinge, Emilieen_US
dc.contributor.authorSagle, Lauraen_US
dc.date.accessioned2016-10-10T14:44:53Zen_US
dc.date.available2016-10-10T14:44:53Zen_US
dc.date.issued2016en_US
dc.description.abstractLipid membranes and membrane proteins are important biosensing targets, motivating the development of label-free methods with improved sensitivity. Silica-coated metal nanoparticles allow these systems to be combined with supported lipid bilayers for sensing membrane proteins through localized surface plasmon resonance (LSPR). However, the small sensing volume of LSPR makes the thickness of the silica layer critical for performance. Here, we develop a simple, inexpensive, and rapid sol–gel method for preparing thin conformal, continuous silica films and demonstrate its applicability using gold nanodisk arrays with LSPRs in the near-infrared range. Silica layers as thin as ∼5 nm are observed using cross-sectional scanning transmission electron microscopy. The loss in sensitivity due to the thin silica coating was found to be only 16%, and the biosensing capabilities of the substrates were assessed through the binding of cholera toxin B to GM1 lipids. This sensor platform should prove useful in the rapid, multiplexed detection and screening of membrane-associated biological targets.en_US
dc.identifier.citationBruzas, Ian, Unser, Sarah, Yazdi, Sadegh, et al.. "Ultrasensitive Plasmonic Platform for Label-Free Detection of Membrane-Associated Species." <i>Analytical Chemistry,</i> 88, no. 16 (2016) American Chemical Society: 7968-7974. http://dx.doi.org/10.1021/acs.analchem.6b00801.en_US
dc.identifier.doihttp://dx.doi.org/10.1021/acs.analchem.6b00801en_US
dc.identifier.urihttps://hdl.handle.net/1911/91773en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the American Chemical Society.en_US
dc.subject.keywordSolid-Supported Lipid Bilayersen_US
dc.subject.keywordplasmonic biosensingen_US
dc.subject.keywordnanodisk arrayen_US
dc.subject.keywordsilica sol-gelen_US
dc.subject.keywordlocalized surface plasmon resonanceen_US
dc.titleUltrasensitive Plasmonic Platform for Label-Free Detection of Membrane-Associated Speciesen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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