Microarray experiments and factors which affect their reliability
| dc.citation.journalTitle | Biology Direct | en_US |
| dc.contributor.author | Jaksik, Roman | en_US |
| dc.contributor.author | Iwanaszko, Marta | en_US |
| dc.contributor.author | Rzeszowska-Wolny, Joanna | en_US |
| dc.contributor.author | Kimmel, Marek | en_US |
| dc.date.accessioned | 2015-09-16T17:51:12Z | en_US |
| dc.date.available | 2015-09-16T17:51:12Z | en_US |
| dc.date.issued | 2015 | en_US |
| dc.date.updated | 2015-09-16T17:51:12Z | en_US |
| dc.description.abstract | Oligonucleotide microarrays belong to the basic tools of molecular biology and allow for simultaneous assessment of the expression level of thousands of genes. Analysis of microarray data is however very complex, requiring sophisticated methods to control for various factors that are inherent to the procedures used. In this article we describe the individual steps of a microarray experiment, highlighting important elements and factors that may affect the processes involved and that influence the interpretation of the results. Additionally, we describe methods that can be used to estimate the influence of these factors, and to control the way in which they affect the expression estimates. A comprehensive understanding of the experimental protocol used in a microarray experiment aids the interpretation of the obtained results. By describing known factors which affect expression estimates this article provides guidelines for appropriate quality control and pre-processing of the data, additionally applicable to other transcriptome analysis methods that utilize similar sample handling protocols. | en_US |
| dc.identifier.citation | Jaksik, Roman, Iwanaszko, Marta, Rzeszowska-Wolny, Joanna, et al.. "Microarray experiments and factors which affect their reliability." <i>Biology Direct,</i> (2015) BioMed Central: http://dx.doi.org/10.1186/s13062-015-0077-2. | en_US |
| dc.identifier.doi | http://dx.doi.org/10.1186/s13062-015-0077-2 | en_US |
| dc.identifier.uri | https://hdl.handle.net/1911/81653 | en_US |
| dc.language.iso | eng | en_US |
| dc.publisher | BioMed Central | en_US |
| dc.rights | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | en_US |
| dc.rights.holder | Jaksik et al. | en_US |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_US |
| dc.title | Microarray experiments and factors which affect their reliability | en_US |
| dc.type | Journal article | en_US |
| dc.type.dcmi | Text | en_US |
| dc.type.publication | publisher version | en_US |