Thermofluidic heat exchangers for actuation of transcription in artificial tissues

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dc.citation.articleNumbereabb9062en_US
dc.citation.issueNumber40en_US
dc.citation.journalTitleScience Advancesen_US
dc.citation.volumeNumber6en_US
dc.contributor.authorCorbett, Daniel C.en_US
dc.contributor.authorFabyan, Wesley B.en_US
dc.contributor.authorGrigoryan, Bagraten_US
dc.contributor.authorO'Connor, Colleen E.en_US
dc.contributor.authorJohansson, Fredriken_US
dc.contributor.authorBatalov, Ivanen_US
dc.contributor.authorRegier, Mary C.en_US
dc.contributor.authorDeForest, Cole A.en_US
dc.contributor.authorMiller, Jordan S.en_US
dc.contributor.authorStevens, Kelly R.en_US
dc.date.accessioned2020-11-06T02:02:48Zen_US
dc.date.available2020-11-06T02:02:48Zen_US
dc.date.issued2020en_US
dc.description.abstractSpatial patterns of gene expression in living organisms orchestrate cell decisions in development, homeostasis, and disease. However, most methods for reconstructing gene patterning in 3D cell culture and artificial tissues are restricted by patterning depth and scale. We introduce a depth- and scale-flexible method to direct volumetric gene expression patterning in 3D artificial tissues, which we call “heat exchangers for actuation of transcription” (HEAT). This approach leverages fluid-based heat transfer from printed networks in the tissues to activate heat-inducible transgenes expressed by embedded cells. We show that gene expression patterning can be tuned both spatially and dynamically by varying channel network architecture, fluid temperature, fluid flow direction, and stimulation timing in a user-defined manner and maintained in vivo. We apply this approach to activate the 3D positional expression of Wnt ligands and Wnt/β-catenin pathway regulators, which are major regulators of development, homeostasis, regeneration, and cancer throughout the animal kingdom.en_US
dc.identifier.citationCorbett, Daniel C., Fabyan, Wesley B., Grigoryan, Bagrat, et al.. "Thermofluidic heat exchangers for actuation of transcription in artificial tissues." <i>Science Advances,</i> 6, no. 40 (2020) AAAS: https://doi.org/10.1126/sciadv.abb9062.en_US
dc.identifier.doihttps://doi.org/10.1126/sciadv.abb9062en_US
dc.identifier.urihttps://hdl.handle.net/1911/109520en_US
dc.language.isoengen_US
dc.publisherAAASen_US
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.en_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/en_US
dc.titleThermofluidic heat exchangers for actuation of transcription in artificial tissuesen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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