Acoustically targeted measurement of transgene expression in the brain

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dc.citation.articleNumbereadj7686en_US
dc.citation.issueNumber32en_US
dc.citation.journalTitleScience Advancesen_US
dc.citation.volumeNumber10en_US
dc.contributor.authorSeo, Joon Pyungen_US
dc.contributor.authorTrippett, James S.en_US
dc.contributor.authorHuang, Zhiminen_US
dc.contributor.authorLee, Sangsinen_US
dc.contributor.authorNouraein, Shirinen_US
dc.contributor.authorWang, Ryan Z.en_US
dc.contributor.authorSzablowski, Jerzy O.en_US
dc.contributor.orgApplied Physics Program;Systems, Synthetic, and Physical Biology Program;Rice Neuroengineering Initiativeen_US
dc.date.accessioned2024-08-29T21:11:50Zen_US
dc.date.available2024-08-29T21:11:50Zen_US
dc.date.issued2024en_US
dc.description.abstractGene expression is a critical component of brain physiology, but monitoring this expression in the living brain represents a major challenge. Here, we introduce a new paradigm called recovery of markers through insonation (REMIS) for noninvasive measurement of gene expression in the brain with cell type, spatial, and temporal specificity. Our approach relies on engineered protein markers that are produced in neurons but exit into the brain’s interstitium. When ultrasound is applied to targeted brain regions, it opens the blood-brain barrier and releases these markers into the bloodstream. Once in blood, the markers can be readily detected using biochemical techniques. REMIS can noninvasively confirm gene delivery and measure endogenous signaling in specific brain sites through a simple insonation and a subsequent blood test. REMIS is reliable and demonstrated consistent improvement in recovery of markers from the brain into the blood. Overall, this work establishes a noninvasive, spatially specific method of monitoring gene delivery and endogenous signaling in the brain.en_US
dc.identifier.citationSeo, J. P., Trippett, J. S., Huang, Z., Lee, S., Nouraein, S., Wang, R. Z., & Szablowski, J. O. (2024). Acoustically targeted measurement of transgene expression in the brain. Science Advances, 10(32), eadj7686. https://doi.org/10.1126/sciadv.adj7686en_US
dc.identifier.digitalsciadv-adj7686en_US
dc.identifier.doihttps://doi.org/10.1126/sciadv.adj7686en_US
dc.identifier.urihttps://hdl.handle.net/1911/117748en_US
dc.language.isoengen_US
dc.publisherAAASen_US
dc.rightsExcept where otherwise noted, this work is licensed under a Creative Commons Attribution (CC BY) license.  Permission to reuse, publish, or reproduce the work beyond the terms of the license or beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleAcoustically targeted measurement of transgene expression in the brainen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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