Systematically probing the bottom-up synthesis of AuPAMAM conjugates for enhanced transfection efficiency

dc.citation.articleNumber24en_US
dc.citation.issueNumber1en_US
dc.citation.journalTitleJournal of Nanobiotechnologyen_US
dc.citation.volumeNumber14en_US
dc.contributor.authorFigueroa, Elizabeth R.en_US
dc.contributor.authorStephen Yan, J.en_US
dc.contributor.authorChamberlain-Simon, Nicolette K.en_US
dc.contributor.authorLin, Adam Y.en_US
dc.contributor.authorFoster, Aaron E.en_US
dc.contributor.authorDrezek, Rebekah A.en_US
dc.date.accessioned2016-08-11T13:49:06Zen_US
dc.date.available2016-08-11T13:49:06Zen_US
dc.date.issued2016en_US
dc.date.updated2016-08-11T13:49:05Zen_US
dc.description.abstractBackground: Gold nanoparticles (AuNPs) have shown great promise as scaffolds for gene therapy vectors due to their attractive physiochemical properties which include biocompatibility, ease of functionalization via the nearly covalent gold-sulfur dative bond, and surface plasmon optical properties. Previously, we synthesized stable AuNP-polyamidoamine (AuPAMAM) conjugates and showed their success in vitro as non-viral gene delivery vectors. Results: In this study, we systematically perturbed each component of the AuPAMAM conjugates and analyzed the resulting effect on transfection efficiency. Due to the modular, bottom-up nature of the AuPAMAM synthesis, we were able to probe each step of the fabrication process. The relationship between each conjugation parameter and the function of the final vector were investigated. More than fourfold enhanced transfection efficiency was achieved by modifying the PAMAM concentration, PAMAM core chemistry, PAMAM terminus chemistry, and self-assembled monolayer composition of the AuPAMAM conjugates. Conclusions: This work suggest that AuPAMAM synthesis platform is a promising non-viral gene therapy approach and highlights the importance of inspecting the role of each individual constituent in all nanotechnology hybrid materials.en_US
dc.identifier.citationFigueroa, Elizabeth R., Stephen Yan, J., Chamberlain-Simon, Nicolette K., et al.. "Systematically probing the bottom-up synthesis of AuPAMAM conjugates for enhanced transfection efficiency." <i>Journal of Nanobiotechnology,</i> 14, no. 1 (2016) BioMed Central: http://dx.doi.org/10.1186/s12951-016-0178-9.en_US
dc.identifier.doihttp://dx.doi.org/10.1186/s12951-016-0178-9en_US
dc.identifier.urihttps://hdl.handle.net/1911/91215en_US
dc.language.isoengen_US
dc.publisherBioMed Centralen_US
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleSystematically probing the bottom-up synthesis of AuPAMAM conjugates for enhanced transfection efficiencyen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
12951_2016_Article_178.pdf
Size:
1.82 MB
Format:
Adobe Portable Document Format
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed upon to submission
Description: