In Vivo Immune Cell Distribution of Gold Nanoparticles in Naïve and Tumor Bearing Mice

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dc.citation.firstpage812
dc.citation.issueNumber4
dc.citation.journalTitleSmall
dc.citation.lastpage819
dc.citation.volumeNumber10
dc.contributor.authorAlmeida, Joao Paulo Mattos
dc.contributor.authorLin, Adam Yuh
dc.contributor.authorLangsner, Robert James
dc.contributor.authorEckels, Phillip
dc.contributor.authorFoster, Aaron Edward
dc.contributor.authorDrezek, Rebekah Anna
dc.date.accessioned2017-06-06T19:07:23Z
dc.date.available2017-06-06T19:07:23Z
dc.date.issued2014
dc.description.abstractGold nanoparticles (AuNP) have been widely used for drug delivery and have recently been explored for applications in cancer immunotherapy. Although AuNPs are known to accumulate heavily in the spleen, the particle distribution within immune cells has not been thoroughly studied. Here, cellular distribution of Cy5 labeled 50 nm AuNPs is characterized within the immune populations of the spleen from naïve and tumor bearing mice using flow cytometry. Surprisingly, approximately 30% of the detected AuNPs are taken up by B cells at 24 h, with about 10% in granulocytes, 18% in dendritic cells, and 8% in T cells. In addition, 3% of the particles are detected within myeloid derived suppressor cells, an immune suppressive population that could be targeted for cancer immunotherapy. Furthermore, it is observed that, over time, the particles traveled from the red pulp and marginal zone to the follicles of the spleen. Taking into consideration that the particle cellular distribution does not change at 1, 6 and 24 h, it is highly suggestive that the immune populations carry the particles and migrate through the spleen instead of the particles migrating through the tissue by cell-cell transfer. Finally, no difference is observed in particle distribution between naïve and tumor bearing mice in the spleen, and nanoparticles are detected within 0.7% of dendritic cells of the tumor microenvironment. Overall, these results can help inform and influence future AuNP delivery design criteria including future applications for nanoparticle-mediated immunotherapy.
dc.identifier.citationAlmeida, Joao Paulo Mattos, Lin, Adam Yuh, Langsner, Robert James, et al.. "In Vivo Immune Cell Distribution of Gold Nanoparticles in Naïve and Tumor Bearing Mice." <i>Small,</i> 10, no. 4 (2014) Wiley: 812-819. https://doi.org/10.1002/smll.201301998.
dc.identifier.doihttps://doi.org/10.1002/smll.201301998
dc.identifier.urihttps://hdl.handle.net/1911/94813
dc.language.isoeng
dc.publisherWiley
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley.
dc.subject.keywordbiodistribution
dc.subject.keywordcancer
dc.subject.keywordgold nanoparticles
dc.subject.keywordimmune system
dc.subject.keywordimmunotherapy
dc.subject.keywordspleen
dc.titleIn Vivo Immune Cell Distribution of Gold Nanoparticles in Naïve and Tumor Bearing Mice
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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