In Vivo Immune Cell Distribution of Gold Nanoparticles in Naïve and Tumor Bearing Mice

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dc.citation.firstpage812en_US
dc.citation.issueNumber4en_US
dc.citation.journalTitleSmallen_US
dc.citation.lastpage819en_US
dc.citation.volumeNumber10en_US
dc.contributor.authorAlmeida, Joao Paulo Mattosen_US
dc.contributor.authorLin, Adam Yuhen_US
dc.contributor.authorLangsner, Robert Jamesen_US
dc.contributor.authorEckels, Phillipen_US
dc.contributor.authorFoster, Aaron Edwarden_US
dc.contributor.authorDrezek, Rebekah Annaen_US
dc.date.accessioned2017-06-06T19:07:23Zen_US
dc.date.available2017-06-06T19:07:23Zen_US
dc.date.issued2014en_US
dc.description.abstractGold nanoparticles (AuNP) have been widely used for drug delivery and have recently been explored for applications in cancer immunotherapy. Although AuNPs are known to accumulate heavily in the spleen, the particle distribution within immune cells has not been thoroughly studied. Here, cellular distribution of Cy5 labeled 50 nm AuNPs is characterized within the immune populations of the spleen from naïve and tumor bearing mice using flow cytometry. Surprisingly, approximately 30% of the detected AuNPs are taken up by B cells at 24 h, with about 10% in granulocytes, 18% in dendritic cells, and 8% in T cells. In addition, 3% of the particles are detected within myeloid derived suppressor cells, an immune suppressive population that could be targeted for cancer immunotherapy. Furthermore, it is observed that, over time, the particles traveled from the red pulp and marginal zone to the follicles of the spleen. Taking into consideration that the particle cellular distribution does not change at 1, 6 and 24 h, it is highly suggestive that the immune populations carry the particles and migrate through the spleen instead of the particles migrating through the tissue by cell-cell transfer. Finally, no difference is observed in particle distribution between naïve and tumor bearing mice in the spleen, and nanoparticles are detected within 0.7% of dendritic cells of the tumor microenvironment. Overall, these results can help inform and influence future AuNP delivery design criteria including future applications for nanoparticle-mediated immunotherapy.en_US
dc.identifier.citationAlmeida, Joao Paulo Mattos, Lin, Adam Yuh, Langsner, Robert James, et al.. "In Vivo Immune Cell Distribution of Gold Nanoparticles in Naïve and Tumor Bearing Mice." <i>Small,</i> 10, no. 4 (2014) Wiley: 812-819. https://doi.org/10.1002/smll.201301998.en_US
dc.identifier.doihttps://doi.org/10.1002/smll.201301998en_US
dc.identifier.urihttps://hdl.handle.net/1911/94813en_US
dc.language.isoengen_US
dc.publisherWileyen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley.en_US
dc.subject.keywordbiodistributionen_US
dc.subject.keywordcanceren_US
dc.subject.keywordgold nanoparticlesen_US
dc.subject.keywordimmune systemen_US
dc.subject.keywordimmunotherapyen_US
dc.subject.keywordspleenen_US
dc.titleIn Vivo Immune Cell Distribution of Gold Nanoparticles in Naïve and Tumor Bearing Miceen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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