Simultaneous and stoichiometric purification of hundreds of oligonucleotides
| dc.citation.articleNumber | 2467 | |
| dc.citation.journalTitle | Nature Communications | |
| dc.citation.volumeNumber | 9 | |
| dc.contributor.author | Pinto, Alessandro | |
| dc.contributor.author | Chen, Sherry X. | |
| dc.contributor.author | Zhang, David Yu | |
| dc.date.accessioned | 2018-09-26T14:52:44Z | |
| dc.date.available | 2018-09-26T14:52:44Z | |
| dc.date.issued | 2018 | |
| dc.description.abstract | Purification of oligonucleotides has traditionally relied on mobility-based separation methods. However, these are imperfect, biased, and difficult to scale high multiplex. Here, we present a method for simultaneous purification of many oligonucleotides that also normalizes concentrations. The method uses a rationally designed randomer capture probe to enrich for oligos with perfect 5' sequences, based on the observation that synthesis errors are correlated: product molecules with one or more deletions in one region are also more likely to have deletions in other regions. Next-generation sequencing analysis of 64-plex 70 nt purification products show a median 78% purity, a significant improvement over polyacrylamide gel electrophoresis and high pressure liquid chromatography (60% median purity). Additionally, 89% of the oligo products are within a factor of 2 of the median concentration. | |
| dc.identifier.citation | Pinto, Alessandro, Chen, Sherry X. and Zhang, David Yu. "Simultaneous and stoichiometric purification of hundreds of oligonucleotides." <i>Nature Communications,</i> 9, (2018) Springer Nature: https://doi.org/10.1038/s41467-018-04870-w. | |
| dc.identifier.digital | s41467-018-04870-w | |
| dc.identifier.doi | https://doi.org/10.1038/s41467-018-04870-w | |
| dc.identifier.uri | https://hdl.handle.net/1911/102718 | |
| dc.language.iso | eng | |
| dc.publisher | Springer Nature | |
| dc.rights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.title | Simultaneous and stoichiometric purification of hundreds of oligonucleotides | |
| dc.type | Journal article | |
| dc.type.dcmi | Text | |
| dc.type.publication | publisher version |
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