Phosphatase activity tunes two-component system sensor detection threshold
| dc.citation.articleNumber | 1433 | en_US |
| dc.citation.journalTitle | Nature Communications | en_US |
| dc.citation.volumeNumber | 9 | en_US |
| dc.contributor.author | Landry, Brian P. | en_US |
| dc.contributor.author | Palanki, Rohan | en_US |
| dc.contributor.author | Dyulgyarov, Nikola | en_US |
| dc.contributor.author | Hartsough, Lucas A. | en_US |
| dc.contributor.author | Tabor, Jeffrey J. | en_US |
| dc.date.accessioned | 2018-08-17T13:36:24Z | en_US |
| dc.date.available | 2018-08-17T13:36:24Z | en_US |
| dc.date.issued | 2018 | en_US |
| dc.description.abstract | Two-component systems (TCSs) are the largest family of multi-step signal transduction pathways in biology, and a major source of sensors for biotechnology. However, the input concentrations to which biosensors respond are often mismatched with application requirements. Here, we utilize a mathematical model to show that TCS detection thresholds increase with the phosphatase activity of the sensor histidine kinase. We experimentally validate this result in engineered Bacillus subtilis nitrate and E. coli aspartate TCS sensors by tuning their detection threshold up to two orders of magnitude. We go on to apply our TCS tuning method to recently described tetrathionate and thiosulfate sensors by mutating a widely conserved residue previously shown to impact phosphatase activity. Finally, we apply TCS tuning to engineer B. subtilis to sense and report a wide range of fertilizer concentrations in soil. This work will enable the engineering of tailor-made biosensors for diverse synthetic biology applications. | en_US |
| dc.identifier.citation | Landry, Brian P., Palanki, Rohan, Dyulgyarov, Nikola, et al.. "Phosphatase activity tunes two-component system sensor detection threshold." <i>Nature Communications,</i> 9, (2018) Springer Nature: https://doi.org/10.1038/s41467-018-03929-y. | en_US |
| dc.identifier.digital | s41467-018-03929-y | en_US |
| dc.identifier.doi | https://doi.org/10.1038/s41467-018-03929-y | en_US |
| dc.identifier.uri | https://hdl.handle.net/1911/102469 | en_US |
| dc.language.iso | eng | en_US |
| dc.publisher | Springer Nature | en_US |
| dc.rights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. | en_US |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_US |
| dc.title | Phosphatase activity tunes two-component system sensor detection threshold | en_US |
| dc.type | Journal article | en_US |
| dc.type.dcmi | Text | en_US |
| dc.type.publication | publisher version | en_US |
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