Recapitulation and Modulation of the Cellular Architecture of a User-Chosen Cell of Interest Using Cell-Derived, Biomimetic Patterning

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dc.citation.firstpage6128en_US
dc.citation.issueNumber6en_US
dc.citation.journalTitleACS Nanoen_US
dc.citation.lastpage6138en_US
dc.citation.volumeNumber9en_US
dc.contributor.authorSlater, John H.en_US
dc.contributor.authorCulver, James C.en_US
dc.contributor.authorLong, Byron L.en_US
dc.contributor.authorHu, Chenyue W.en_US
dc.contributor.authorHu, Jingzheen_US
dc.contributor.authorBirk, Taylor F.en_US
dc.contributor.authorQutub, Amina A.en_US
dc.contributor.authorDickinson, Mary E.en_US
dc.contributor.authorWest, Jennifer L.en_US
dc.date.accessioned2017-05-15T17:23:58Zen_US
dc.date.available2017-05-15T17:23:58Zen_US
dc.date.issued2015en_US
dc.description.abstractHeterogeneity of cell populations can confound population-averaged measurements and obscure important findings or foster inaccurate conclusions. The ability to generate a homogeneous cell population, at least with respect to a chosen trait, could significantly aid basic biological research and development of high-throughput assays. Accordingly, we developed a high-resolution, image-based patterning strategy to produce arrays of single-cell patterns derived from the morphology or adhesion site arrangement of user-chosen cells of interest (COIs). Cells cultured on both cell-derived patterns displayed a cellular architecture defined by their morphology, adhesive state, cytoskeletal organization, and nuclear properties that quantitatively recapitulated the COIs that defined the patterns. Furthermore, slight modifications to pattern design allowed for suppression of specific actin stress fibers and direct modulation of adhesion site dynamics. This approach to patterning provides a strategy to produce a more homogeneous cell population, decouple the influences of cytoskeletal structure, adhesion dynamics, and intracellular tension on mechanotransduction-mediated processes, and a platform for high-throughput cellular assays.en_US
dc.identifier.citationSlater, John H., Culver, James C., Long, Byron L., et al.. "Recapitulation and Modulation of the Cellular Architecture of a User-Chosen Cell of Interest Using Cell-Derived, Biomimetic Patterning." <i>ACS Nano,</i> 9, no. 6 (2015) American Chemical Society: 6128-6138. http://dx.doi.org/10.1021/acsnano.5b01366.en_US
dc.identifier.doihttp://dx.doi.org/10.1021/acsnano.5b01366en_US
dc.identifier.urihttps://hdl.handle.net/1911/94266en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the American Chemical Society.en_US
dc.subject.keywordlaser scanning lithographyen_US
dc.subject.keywordimage-guided patterningen_US
dc.subject.keywordbiomimeticen_US
dc.subject.keywordcell-derived patterningen_US
dc.subject.keywordnanopatterningen_US
dc.subject.keywordself-assembled monolayersen_US
dc.subject.keywordactin cytoskeletonen_US
dc.subject.keywordcell adhesionen_US
dc.subject.keywordmechanotransductionen_US
dc.subject.keywordcell patterningen_US
dc.subject.keywordcell population heterogeneityen_US
dc.subject.keywordcell population homogeneityen_US
dc.subject.keywordcell engineeringen_US
dc.subject.keywordcell arraysen_US
dc.titleRecapitulation and Modulation of the Cellular Architecture of a User-Chosen Cell of Interest Using Cell-Derived, Biomimetic Patterningen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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