Biochar and Microbial Signaling: Production Conditions Determine Effects on Microbial Communication

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dc.citation.firstpage11496en_US
dc.citation.issueNumber20en_US
dc.citation.journalTitleEnvironmental Science & Technologyen_US
dc.citation.lastpage11503en_US
dc.citation.volumeNumber47en_US
dc.contributor.authorMasiello, Caroline A.en_US
dc.contributor.authorChen, Yeen_US
dc.contributor.authorGao, Xiaodongen_US
dc.contributor.authorLiu, Shirleyen_US
dc.contributor.authorCheng, Hsiao-Yingen_US
dc.contributor.authorBennett, Matthew R.en_US
dc.contributor.authorRudgers, Jennifer A.en_US
dc.contributor.authorWagner, Daniel S.en_US
dc.contributor.authorZygourakis, Kyriacosen_US
dc.contributor.authorSilberg, Jonathan J.en_US
dc.date.accessioned2015-07-09T20:14:49Zen_US
dc.date.available2015-07-09T20:14:49Zen_US
dc.date.issued2013en_US
dc.description.abstractCharcoal has a long soil residence time, which has resulted in its production and use as a carbon sequestration technique (biochar). A range of biological effects can be triggered by soil biochar that can positively and negatively influence carbon storage, such as changing the decomposition rate of organic matter and altering plant biomass production. Sorption of cellular signals has been hypothesized to underlie some of these effects, but it remains unknown whether the binding of biochemical signals occurs, and if so, on time scales relevant to microbial growth and communication. We examined biochar sorption of N-3-oxo-dodecanoyl-L-homoserine lactone, an acyl-homoserine lactone (AHL) intercellular signaling molecule used by many gram-negative soil microbes to regulate gene expression. We show that wood biochars disrupt communication within a growing multicellular system that is made up of sender cells that synthesize AHL and receiver cells that express green fluorescent protein in response to an AHL signal. However, biochar inhibition of AHL-mediated cell–cell communication varied, with the biochar prepared at 700 °C (surface area of 301 m2/g) inhibiting cellular communication 10-fold more than an equivalent mass of biochar prepared at 300 °C (surface area of 3 m2/g). These findings provide the first direct evidence that biochars elicit a range of effects on gene expression dependent on intercellular signaling, implicating the method of biochar preparation as a parameter that could be tuned to regulate microbial-dependent soil processes, like nitrogen fixation and pest attack of root crops.en_US
dc.identifier.citationMasiello, Caroline A., Chen, Ye, Gao, Xiaodong, et al.. "Biochar and Microbial Signaling: Production Conditions Determine Effects on Microbial Communication." <i>Environmental Science & Technology,</i> 47, no. 20 (2013) American Chemical Society: 11496-11503. http://dx.doi.org/10.1021/es401458s.en_US
dc.identifier.doihttp://dx.doi.org/10.1021/es401458sen_US
dc.identifier.urihttps://hdl.handle.net/1911/80870en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the American Chemical Society.en_US
dc.subject.keywordbiocharen_US
dc.subject.keywordcarbon sequestrationen_US
dc.subject.keywordcharcoalen_US
dc.subject.keywordmicrobial communicationen_US
dc.titleBiochar and Microbial Signaling: Production Conditions Determine Effects on Microbial Communicationen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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