Compensatory Mutations in Predicted Metal Transporters Modulate Auxin Conjugate Responsiveness in Arabidopsis

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dc.citation.firstpage131en_US
dc.citation.journalTitleG3: Genes, Genomics, Geneticsen_US
dc.citation.lastpage141en_US
dc.citation.volumeNumber3en_US
dc.contributor.authorRampey, Rebekah A.en_US
dc.contributor.authorBaldridge, Megan T.en_US
dc.contributor.authorFarrow, David C.en_US
dc.contributor.authorBay, Sarah N.en_US
dc.contributor.authorBartel, Bonnieen_US
dc.date.accessioned2013-01-17T20:51:43Zen_US
dc.date.available2013-01-17T20:51:43Zen_US
dc.date.issued2013-01en_US
dc.description.abstractLevels of the phytohormone indole-3-acetic acid (IAA) can be altered by the formation and hydrolysis of IAA conjugates. The isolation and characterization of Arabidopsis thaliana mutants with reduced IAA-conjugate sensitivity and wild-type IAA responses is advancing the understanding of auxin homeostasis by uncovering the factors needed for conjugate metabolism. For example, the discovery that the IAA-Ala-resistant mutant iar1 is defective in a protein in the ZIP family of metal transporters uncovered a link between metal homeostasis and IAA-conjugate sensitivity. To uncover additional factors impacting auxin conjugate metabolism, we conducted a genetic modifier screen and isolated extragenic mutations that restored IAA-amino acid conjugate sensitivity to the iar1 mutant. One of these suppressor mutants is defective in a putative cation diffusion facilitator, MTP5 (At3g12100; formerly known as MTPc2). Loss of MTP5 function restored IAA conjugate sensitivity to iar1 but not to mutants defective in IAA-amino acid conjugate amidohydrolases. Our results are consistent with a model in which MTP5 and IAR1 transport metals in an antagonistic fashion to regulate metal homeostasis within the subcellular compartment in which the IAA-conjugate amidohydrolases reside, and support previous suggestions that the ion composition in this compartment influences hydrolase activity.en_US
dc.embargo.termsnoneen_US
dc.identifier.citationRampey, Rebekah A., Baldridge, Megan T., Farrow, David C., et al.. "Compensatory Mutations in Predicted Metal Transporters Modulate Auxin Conjugate Responsiveness in Arabidopsis." <i>G3: Genes, Genomics, Genetics,</i> 3, (2013) The Genetics Society of America: 131-141. http://dx.doi.org/10.1534/g3.112.004655.en_US
dc.identifier.doihttp://dx.doi.org/10.1534/g3.112.004655en_US
dc.identifier.urihttps://hdl.handle.net/1911/69835en_US
dc.language.isoengen_US
dc.publisherThe Genetics Society of Americaen_US
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/en_US
dc.titleCompensatory Mutations in Predicted Metal Transporters Modulate Auxin Conjugate Responsiveness in Arabidopsisen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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