Compensatory Mutations in Predicted Metal Transporters Modulate Auxin Conjugate Responsiveness in Arabidopsis

Simple item page
dc.citation.firstpage131
dc.citation.journalTitleG3: Genes, Genomics, Genetics
dc.citation.lastpage141
dc.citation.volumeNumber3
dc.contributor.authorRampey, Rebekah A.
dc.contributor.authorBaldridge, Megan T.
dc.contributor.authorFarrow, David C.
dc.contributor.authorBay, Sarah N.
dc.contributor.authorBartel, Bonnie
dc.date.accessioned2013-01-17T20:51:43Z
dc.date.available2013-01-17T20:51:43Z
dc.date.issued2013-01
dc.description.abstractLevels of the phytohormone indole-3-acetic acid (IAA) can be altered by the formation and hydrolysis of IAA conjugates. The isolation and characterization of Arabidopsis thaliana mutants with reduced IAA-conjugate sensitivity and wild-type IAA responses is advancing the understanding of auxin homeostasis by uncovering the factors needed for conjugate metabolism. For example, the discovery that the IAA-Ala-resistant mutant iar1 is defective in a protein in the ZIP family of metal transporters uncovered a link between metal homeostasis and IAA-conjugate sensitivity. To uncover additional factors impacting auxin conjugate metabolism, we conducted a genetic modifier screen and isolated extragenic mutations that restored IAA-amino acid conjugate sensitivity to the iar1 mutant. One of these suppressor mutants is defective in a putative cation diffusion facilitator, MTP5 (At3g12100; formerly known as MTPc2). Loss of MTP5 function restored IAA conjugate sensitivity to iar1 but not to mutants defective in IAA-amino acid conjugate amidohydrolases. Our results are consistent with a model in which MTP5 and IAR1 transport metals in an antagonistic fashion to regulate metal homeostasis within the subcellular compartment in which the IAA-conjugate amidohydrolases reside, and support previous suggestions that the ion composition in this compartment influences hydrolase activity.
dc.embargo.termsnone
dc.identifier.citationRampey, Rebekah A., Baldridge, Megan T., Farrow, David C., et al.. "Compensatory Mutations in Predicted Metal Transporters Modulate Auxin Conjugate Responsiveness in Arabidopsis." <i>G3: Genes, Genomics, Genetics,</i> 3, (2013) The Genetics Society of America: 131-141. http://dx.doi.org/10.1534/g3.112.004655.
dc.identifier.doihttp://dx.doi.org/10.1534/g3.112.004655
dc.identifier.urihttps://hdl.handle.net/1911/69835
dc.language.isoeng
dc.publisherThe Genetics Society of America
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/
dc.titleCompensatory Mutations in Predicted Metal Transporters Modulate Auxin Conjugate Responsiveness in Arabidopsis
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Compensatory-Mutations.pdf
Size:
4.05 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Biochemistry and Cell Biology Publications
Faculty Publications