Extending single molecule fluorescence observation time by amplitude-modulated excitation

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dc.citation.firstpage37001
dc.citation.issueNumber3
dc.citation.journalTitleMethods and Applications in Fluorescence
dc.citation.volumeNumber1
dc.contributor.authorKisley, Lydia
dc.contributor.authorChang, Wei-Shun
dc.contributor.authorCooper, David
dc.contributor.authorMansur, Andrea P.
dc.contributor.authorLandes, Christy F.
dc.date.accessioned2015-07-10T15:47:00Z
dc.date.available2015-07-10T15:47:00Z
dc.date.issued2013
dc.description.abstractWe present a hardware-based method that can improve single molecule fluorophore observation time by up to 1500% and super-localization by 47% for the experimental conditions used. The excitation was modulated using an acousto-optic modulator (AOM) synchronized to the data acquisition and inherent data conversion time of the detector. The observation time and precision in super-localization of four commonly used fluorophores were compared under modulated and traditional continuous excitation, including direct total internal reflectance excitation of Alexa 555 and Cy3, non-radiative Förster resonance energy transfer (FRET) excited Cy5, and direct epi-fluorescence wide field excitation of Rhodamine 6G. The proposed amplitude-modulated excitation does not perturb the chemical makeup of the system or sacrifice signal and is compatible with multiple types of fluorophores. Amplitude-modulated excitation has practical applications for any fluorescent study utilizing an instrumental setup with time-delayed detectors.
dc.identifier.citationKisley, Lydia, Chang, Wei-Shun, Cooper, David, et al.. "Extending single molecule fluorescence observation time by amplitude-modulated excitation." <i>Methods and Applications in Fluorescence,</i> 1, no. 3 (2013) IOP Publishing: 37001. http://dx.doi.org/10.1088/2050-6120/1/3/037001.
dc.identifier.doihttp://dx.doi.org/10.1088/2050-6120/1/3/037001
dc.identifier.urihttps://hdl.handle.net/1911/80874
dc.language.isoeng
dc.publisherIOP Publishing
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by IOP Publishing.
dc.titleExtending single molecule fluorescence observation time by amplitude-modulated excitation
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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