Extending single molecule fluorescence observation time by amplitude-modulated excitation

Simple item page
dc.citation.firstpage37001en_US
dc.citation.issueNumber3en_US
dc.citation.journalTitleMethods and Applications in Fluorescenceen_US
dc.citation.volumeNumber1en_US
dc.contributor.authorKisley, Lydiaen_US
dc.contributor.authorChang, Wei-Shunen_US
dc.contributor.authorCooper, Daviden_US
dc.contributor.authorMansur, Andrea P.en_US
dc.contributor.authorLandes, Christy F.en_US
dc.date.accessioned2015-07-10T15:47:00Zen_US
dc.date.available2015-07-10T15:47:00Zen_US
dc.date.issued2013en_US
dc.description.abstractWe present a hardware-based method that can improve single molecule fluorophore observation time by up to 1500% and super-localization by 47% for the experimental conditions used. The excitation was modulated using an acousto-optic modulator (AOM) synchronized to the data acquisition and inherent data conversion time of the detector. The observation time and precision in super-localization of four commonly used fluorophores were compared under modulated and traditional continuous excitation, including direct total internal reflectance excitation of Alexa 555 and Cy3, non-radiative Förster resonance energy transfer (FRET) excited Cy5, and direct epi-fluorescence wide field excitation of Rhodamine 6G. The proposed amplitude-modulated excitation does not perturb the chemical makeup of the system or sacrifice signal and is compatible with multiple types of fluorophores. Amplitude-modulated excitation has practical applications for any fluorescent study utilizing an instrumental setup with time-delayed detectors.en_US
dc.identifier.citationKisley, Lydia, Chang, Wei-Shun, Cooper, David, et al.. "Extending single molecule fluorescence observation time by amplitude-modulated excitation." <i>Methods and Applications in Fluorescence,</i> 1, no. 3 (2013) IOP Publishing: 37001. http://dx.doi.org/10.1088/2050-6120/1/3/037001.en_US
dc.identifier.doihttp://dx.doi.org/10.1088/2050-6120/1/3/037001en_US
dc.identifier.urihttps://hdl.handle.net/1911/80874en_US
dc.language.isoengen_US
dc.publisherIOP Publishingen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by IOP Publishing.en_US
dc.titleExtending single molecule fluorescence observation time by amplitude-modulated excitationen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
nihms-509067.pdf
Size:
1.73 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Faculty Publications
Chemistry Publications
ECE Publications