Design of a Fast, Efficient and Controlled DNA Shearing System Based on Lateral Acoustic Waves

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dc.contributor.advisorMassoud, Yehia
dc.creatorDev, Kapil
dc.date.accessioned2013-03-08T00:33:20Z
dc.date.available2013-03-08T00:33:20Z
dc.date.issued2012
dc.description.abstractWith the continuous research and advances in Deoxyribonucleic acid (DNA) sequencing technologies, the need for an efficient DNA shearing system has increased more than ever before. In this thesis, we propose a fast, efficient and controlled DNA shearing system based on a uniquely designed ultrasonic transducer, called Fresnel Annular Sector Actuator (FASA). Based on the simulation and experimental results, a circular array of four 90°-FASA elements is chosen as the basic unit for the proposed shearing system. DNA is successfully sheared from 300 to 1500 base-pair lengths. The shearing performance of the system is independent of the source of DNA over a large range of concentrations of the DNA. Finally, multiple FASA elements, excited by separate BF-signals, are used to increase the throughput of the proposed shearing system.
dc.format.extent96 p.en_US
dc.format.mimetypeapplication/pdf
dc.identifier.callnoTHESIS E.E. 2012 DEV
dc.identifier.citationDev, Kapil. "Design of a Fast, Efficient and Controlled DNA Shearing System Based on Lateral Acoustic Waves." (2012) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/70227">https://hdl.handle.net/1911/70227</a>.
dc.identifier.digitalDevKen_US
dc.identifier.urihttps://hdl.handle.net/1911/70227
dc.language.isoeng
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.
dc.subjectApplied sciences
dc.subjectBiomedical engineering
dc.subjectElectrical engineering
dc.titleDesign of a Fast, Efficient and Controlled DNA Shearing System Based on Lateral Acoustic Waves
dc.typeThesis
dc.type.materialText
thesis.degree.departmentElectrical Engineering
thesis.degree.disciplineEngineering
thesis.degree.grantorRice University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science
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